Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 292-282-9 | CAS number: 90583-79-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- other: Read-across information from structurally closely related substance according to REACH Annex XI, 1.5.
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with detailed documentation (read-across: study conducted with a similar substance)
- Justification for type of information:
- See attached read-across justification
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Fraunhofer IME
Test material
- Reference substance name:
- Glycine, N-coco acyl derivs., potassium salts
- Cas Number:
- 301341-58-2
- Molecular formula:
- R-CO-NH-CH2-COOK (Please note: "R-CO-" is a an acyl residue (cocoyl)
- IUPAC Name:
- Glycine, N-coco acyl derivs., potassium salts
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: 61.81 SAG
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Göttingen, Germany
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.5 - 22.0 °C
- pH:
- Control: 8.31 at test start, 8.17 – 8.63 at test end
Test cultures: initial pH 7.84 - 8.35, at test end 7.95 – 8.61 - Nominal and measured concentrations:
- 50.0, 25.0, 12.5, 6.25 and 3.13 mg/L (nominal concentrations)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL conical glass flasks
- Type:covered with air-permeable siliconesponge caps
- Initial cells density: 10 000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: pH measured in one test vessel per test concentration including the control. At the end of the test, the solution pH values were measured directly in test vessels. The test culture temperature was measured in one test vessel only at test start
OTHER TEST CONDITIONS
- Photoperiod: continuous illimination
- Light intensity and quality: 8127 - 8221 lux
EFFECT PARAMETERS MEASURED:
Cell concentrations were determined with an electronic particle counter at test start and after 24, 48 and 72 h - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 14.0 - 17.0 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CI 5.87 - 6.79 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 3.50 - 5.35 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.98 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CI 2.49 - 3.40 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and yield
- Results with reference substance (positive control):
- ErC50 2.91 mg/L (95% CI 2.35 - 3.63 mg/L)
- Reported statistics and error estimates:
- EC50, EC20 and EC10: Probit-analysis assuming log-normal distribution
NOEC and LOEC: ANOVA followed by Williams Multiple Sequential t-test
Computer program ToxRat used for the statistical evaluations
Any other information on results incl. tables
Growth inhibition of 3.10, 11.6, 46.8, 72.5 and 80.7 % was observed at 3.13, 6.25, 12.5, 25.0 and 50.0 mg/L respectively. Yield inhibition of 14.2, 45.6, 91.6, 98.2 and 99.0 % was observed at 3.13, 6.25, 12.5, 25.0 and 50.0 mg/L respectively. There were no compound related visual phytotoxic effects.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of the test item to Pseudokirchnerella subcapitata was investigated in a study conducted according to OECD Guideline 201. The 72 h EC50 (based on growth rate) was determined to be 15.4 mg/L based on nominal concentration.
- Executive summary:
The toxicity of the test item to Pseudokirchnerella subcapitata was investigated in a study conducted according to OECD Guideline 201. The 72 h EC50 (based on growth rate) was determined to be 15.4 mg/L based on nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
