1,1-dimethylprop-3-ynylamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-09-15 to 1999-09-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 1984

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

Public Draft, April 1996

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

EEC Directive 92/32/EEC (December 1992), Annex V, part C: Methods for Determination of Ecotoxicity. C.2. Acute Toxicity to Daphnia

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Qualifier:

according to guideline

Guideline:

other: International Standard ISO/DIS 10706 Water quality - Determination of long term toxicity of substances to Daphnia magna STRAUS

GLP compliance:

yes

Remarks:

registered for GLP under the name "BASF DUU" in German Federal Gazette No. 140 page 8633 of 30 July 1996.

Specific details on test material used for the study:

- Purity: not specified
- Date of production: 1999-03-02
- Date of production: 1999-03-02
- Date of receipt of test substance: 1999-09-15
- Batch No.:Abl.Nr.99-0031
- Physical state: liquid
- Colour: colourless-yellowish
- Water-solubility: optionally miscible
- Homogeneity: yes
- Instability against: oxigene
- Special storage conditions: under N2
- Storage conditions before start of test: refrigerator

Analytical monitoring:

yes

Details on sampling:

- Date of sample preparation and sampling: 1999-09-15
- Number of replicates for the measurement of pH and Oxygen: 1
- Number of parallels without animals: 1 (parallel 5) per test concentration and control for measurement of the pH and oxygen content at the beginning of the test. This parallel was thrown away after measurement. 2 per test concentration and control for concentration control analysis.
- Check of the study and recording (swimming ability): Visually after 0, 24 and 48 h

For calibration, the test substance was weighed in a volumetric ftask and dissolved in hydrochloric acid (4%) and made up to volume using demin. water. Two drops of hydrochloric acid (4%) were added to the sample solutions before derivatization.

Vehicle:

no

Details on test solutions:

- Synthetic fresh water: used for culture and test purposes
- M4 medium (according to ISO 10706), conductivity < 0.05 μS/cm
- Total hardness: 2.20 - 3.20 mmol/I
- Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/I
- Molar ratio Ca:Mg about 4: 1
- pH value: 7.5 - 8.5
- Conductivity: 550 - 650 μS/cm
- Aerated for approximately 24 h until saturation with oxygen was reached
- Preparation of the stock solution and dilution: With an Eppendorf-pipete the test substance was directly given (122 μI) into a 1 1- flask with M4-medium.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: In-house culture; original culture was obtained from Institut National de Recherche Chimique Appliquee, France, in 1978
- Age of the animals at the start of the test: 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals: 2 - 4 weeks

Test type:

static

Water media type:

freshwater

Remarks:

synthetic freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

Total hardness: 2.20 - 3.20 mmol/L

Test temperature:

20.4 °C

pH:

7.5 - 8.5

Dissolved oxygen:

7.9 - 8.9 mg/L

Salinity:

not applicable

Conductivity:

550 - 650 μS/cm

Nominal and measured concentrations:

Nominal concentration test item: 0 (control), 100 mg/L
Measured concentration test item: 92 - 94 mg/L

Details on test conditions:

- Alkalinity: Up to pH 4.3: 0,80 - 1,00 mmol/L
- Test vessel: Glass tubes sealed with gas unpermeable teflone caps (nominal volume 20 ml)
- Test volume: Appr. 23 ml (the vials were filled close to the bottom)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Loading (animals/ml): 0.2
- Number of animals/vessel: 5
- Total number of animals/conc.: 20
- Illumination: Artificial light, type warm white (e.g. OSRAM L58 W31) day:night rhythm = 16 : 8 h
- Light intensity: About 2 - 7 μE/(m2·s) at a wave length of 400 - 700 nm
- Test parameter: Swimming ability of the test animals
- Feeding: None
- Measurement of temperature: Continuously du ring the whole test period in a separate vessel close to the test vessels.
- Measurement of pH and oxygen: After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration

Reference substance (positive control):

yes

Remarks:

The EC50(24h) opotassium dichromate (Date of the control experiment: 18 Aug 1999, project number: 97/0242/50/36)

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- In the control the immobilization was <= 10%
- In the control no daphnids were captured in a surface film
- The pH-value in the test solutions did not vary within more than one unit
- Effect concentrations exceeding solubility of substance in test medium: No
- The EC50(24h) of the control substance potassium dichromate was 0,68 mg/I (Date of the last control experiment: 18 Aug 1999, project number: 97/0242/50/36
- Immobilization: Based on the nominal concentrations of the test substance

Results with reference substance (positive control):

The EC50 (24h) of the control substance potassium dichromate was 0,68 mg/I (Date of the last control experiment: 18 Aug 1999, project number: 97/0242/50/36).

Reported statistics and error estimates:

The test results allowed no statistical evaluation of the data.

Number of mobile Daphnids in the test concentration (100 mg/I) and the control.

Conc.	Parallel 1			Parallel 2			Parallel 3			Parallel 4			Sum
[mg/L]	[h]			[h]			[h]			[h]			[h]
	0	24	48	0	24	48	0	24	48	0	24	48	24	48
control	5	5	5	5	5	5	5	5	5	5	5	5	20	20
100	5	5	4	5	5	5	5	5	5	5	5	5	20	19

Validity criteria fulfilled:

yes

Description of key information

The EC50 value of the test item in aquatic invertebrates was determined to be > 100 mg/L after 48 hours exposure.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute immobilisation of the test item to Daphnia magna (STRAUS) was determined under GLP conditions according to EEC Directive 92/32/EEC, Method C.2. (December 1992), OECD Guideline 202 (April 1984), EPA OPPTS 850.1010 (Public Draft, April 1996I), ISO 6341: 1989 and ISO/DIS 10706. The test was performed in a static and closed system as a limit-test over a duration of 48 hours with the only nominal test concentration of 100 mg/L (measured concentration: 92 - 94 mg/L), along with a laboratory negative control (0 mg/L). Four replicates (with five Daphnids) each for the test concentration and the negative controls were used. A positive control was performed on 18 Aug 1999 (BASF project number: 97/0242/50/36), using the reference substance potassium dichromat.

The test met validity criteria with 0% of the control organisms showing immobilisation, signs of disease, stress, or unusual behaviour, and the minimum dissolved oxygen concentration in the test solutions at the end of the test was >3 mg O2/L (8.9 – 9.0 mg O2/L). The water quality measurements (e.g., temperature, pH) were also within the tolerance limits of the test organism. The test endpoint was based on the nominal concentration of the test item since the measured concentrations of the test item were within 80% to 120% of the nominal concentration. The EC50(24h) of the control substance potassium dichromate was 0,68 mg/I. Based on the nominal concentrations of the test substance the 48-h EC50 was determined to be > 100 mg/L. The test item is considered to be not toxic to aquatic invertebrates as no aquatic toxicity was observed at 100 mg/L within range of water solubility.