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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21 November 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
2010.07.22
Deviations:
no
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Version / remarks:
2007.02.01
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Manufacturing date: June 2017
Expiry date: June 2019
Lot no. 70616121
Form: Clear viscous liquid
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Activated sludge collected from Nivå WWTP:
32 g/L SS adjusted with tap water to approximately 24 g/L SS.
Sludge concentration in the test mixtures were 2.31 to 2.41 g SS/L
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min
Test temperature:
Room temperature: appr. 22 °C
The temperature registered during oxygen measurements was in the range of 20.0-21.5 °C
Nominal and measured concentrations:
Nominal concentrations: 0 (control),10, 20, 50, 100 and 200 mg/L
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol (analytical grade)
Key result
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Results with reference substance (positive control):
EC20 (30 min) = 2.0 mg/L
EC50 (30 min) = 10.4 mg/L
EC20 (180 min) = 0.8 (0.6-0.9) mg/L
EC5 0 (180 min) = 7.0 (6.6-7.5 mg/L)
Validity criteria fulfilled:
no
Remarks:
The validity criterion of an oxygen uptake rate ≥ 20 mg O2/L of dry weight sludge per hour was not fulfilled (rate 19.6 mg O2/L). However, since the preliminary screening of the respiration rate by the inoculum was valid, the test is considered valid.
Conclusions:
The effect of the test item on the total respiration (covering respiration by nitrifying organisms and respiration by heterotrophic microorganisms) of activated sludge microorganisms was tested according to OECD guideline 209 and ISO 8192.
The following results were obtained:
EC20 (30 min) > 200 mg/L
EC50 (30 min) > 200 mg/L
EC20 (180 min) > 200 mg/L
EC50 (180 min) > 200 mg/L
Executive summary:

The toxicity to microorganisms of the substance reaction product of the esterification of diglycerol with decanoic acid was studied in an activated sludge, respiration inhibition test carried out in compliance with GLP. The effect of the test item (PGLCP 102 KC) on the total respiration (covering respiration by nitrifying organisms and respiration by heterotrophic microorganisms) of activated sludge microorganisms was tested according to OECD guideline 209 and ISO 8192. The following concentrations were applied: 0 (control), 10, 20, 50, 100 and 200 mg/L.

The following results were obtained:

EC20 (30 min) > 200 mg/L

EC50 (30 min) > 200 mg/L

EC20 (180 min) > 200 mg/L

EC50 (180  min) > 200 mg/L

Description of key information

In the activated sludge, respiration inhibition test, the following results were obtained:

EC20 (30 min) > 200 mg/L

EC50 (30 min)  > 200 mg/L

EC20 (180 min)  > 200 mg/L

EC50 (180  min)  > 200 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
200 mg/L
EC10 or NOEC for microorganisms:
200 mg/L

Additional information