Registration Dossier
Registration Dossier
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EC number: 269-284-3 | CAS number: 68214-04-0
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Toxicity studies on native Procion Red HE-3B and released dye from affinity material exposed to degradative chemical conditions
- Author:
- N. Sdiqui et al.
- Year:
- 1 994
- Bibliographic source:
- J. Biochem. Biophys. Methods 29 (1994) 269-252
- Report date:
- 1994
- Reference Type:
- publication
- Title:
- Etudes de toxicite in vitro et in vivo de colorants couramment utilises en chromatographie d'affinite
- Author:
- P Santambien et al.
- Year:
- 1 994
- Bibliographic source:
- Ann. pharmaceutiques francaises 1994, 52, no 3, p. 137-152
- Report date:
- 1993
Materials and methods
- GLP compliance:
- no
- Endpoint addressed:
- other: cytotoxicity in MRC-5 human cells and HELA cells
Test material
- Reference substance name:
- Hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]
- EC Number:
- 269-284-3
- EC Name:
- Hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]
- Cas Number:
- 68214-04-0
- Molecular formula:
- C44H24Cl2N14Na6O20S6
- IUPAC Name:
- hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- no details in the publication (described as native)
Results and discussion
- Details on results:
- exp 1: The cell growth was not modified significantly and cell division ratio was very similar to controls. The average numbers of cells cultured in the presence of RR120 showed a growth of respectively 101% .
No changes in cell morphology were observed. No polypoidy was found in more than 2,000 metaphases per culture.
exp 2: The dose/effect trials done in microwell plates over 24 h showed no negative effect of the dyes in the cell culture medium; cell viability was very good even for large amounts of RR120
Applicant's summary and conclusion
- Conclusions:
- No cytotoxicity observed in human MRC-5 cells and Hela cells
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