2-phenylethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

SCREENING OF TOBACCO SMOKE CONSTITUENTS FOR MUTAGENICITY USING THE AMES' TEST

Author:

Florin, I., Rutberg, L., Curvall, M. and Enzell, C.R.

Year:

1980

Bibliographic source:

Toxicology, 18 (1980) 219-232

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-phenylethanol

EC Number:

200-456-2

EC Name:

2-phenylethanol

Cas Number:

60-12-8

Molecular formula:

C8H10O

IUPAC Name:

2-phenylethanol

Test material form:

liquid

Method

Target gene:

TA98, TA100, TA1537, TA98 and TA100

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

other: histadine requiring

Metabolic activation:

with and without

Test concentrations with justification for top dose:

3 µmol/plate

Vehicle / solvent:

Spectroscopic grade ethanol

Controls

Positive controls:

yes

Positive control substance:

N-ethyl-N-nitro-N-nitrosoguanidine

other: 2-aminoanthracene

Details on test system and experimental conditions:

The S-9 mix contained per ml: 100 µmol sodium phosphate buffer (pH 7.4), 8 µmol MgC12, 33 µmol KC1, 5 µmol glucose 6-phosphate, 4 µmol NADP, and 0.03-0.1 ml S-9.

Statistics:

Not specified.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: All strains (TA 1535, TA 1537, TA 98 and TA 100) tested

Applicant's summary and conclusion

Conclusions:

2-phenylethanol is not mutagenic.

Executive summary:

The mutagenic activity of 239 individual components of tobacco smoke (including 2-phenylethanol) was assessed using the Ames test. The bacterial strains TA 1535, TA 98 and TA100 of S. typhimurium were used with and without metabolic activation using liver fraction S-9. 2 -phenylethanol was shown to be not mutagenic.