Absolute of Cistus ladaniferus (Cistaceae) obtained from labdanum concrete by ethanol extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10-13 October 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 13-14 October 2014 / signed on 8 April 2015

Type of method:

pycnometer method

Dynamic viscosity of test material:

It was checked if the test item was flowable and if it was possible to inject the test item with a one way syringe.

Key result

Type:

density

Density:

1.023 g/cm³

Temp.:

20 °C

Key result

Type:

relative density

Density:

1.023 other: unitless

Temp.:

20 °C

Pre-test for viscosity

The test item was flowable and it was possible to inject the test item with a one way syringe.

This pre- test was done on 10th Oct. 2017

Table 4.4/1: Measured Values

 

First Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3
Item	Pycn. empty	Pycn. + H2O	Pycn. + test item
Mass in g	36.2273	61.7718	62.4311
Temperature in °C	20.2	20.0	20.4
Volume of Pycnometer	25.5905	cm^3
				Error rel.	Error abs.
Density of Test Item	1.0240	g/cm^3		0.0046%	0.0000
Second Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3
Item	Pycn. empty	Pycn. + H2O	Pycn. + test item
Mass in g	36.2279	61.7757	62.4000
Temperature in °C	20.0	20.0	20.2
Volume of Pycnometer	25.5938	cm^3
				Error rel.	Error abs.
Density of Test Item	1.0226	g/cm^3		0.0046%	0.0000

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.0240	0.0000
2	1.0226	0.0000

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	25.59	cm^3	0.0023	0.01%
Density of Test Item	1.0233	g/ cm^3	0.0010	0.09%
Density of Test Item	1023.3	kg/m^3	1.0	0.09%
Relative Density of Test Item (D20/4)	1.0233		0.0010	0.09%

The density of the test item was calculated as 1.0233 ± 0.0010 g/cm³ at 20.0 ± 0.4 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was measured as 1.0233 ± 0.0010 g/cm3 at 20.0 ± 0.4 °C and the relative density is determined as D20/4 = 1.0233
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density.

Mean density: 1.0233 g/cm³at 20.0 ± 0.4 °C

Mean relative density (D20/4) : 1.0233

Description of key information

The density of the test item was calculated as 1.0233 ± 0.0010 g/cm3 at 20.0 ± 0.4 °C and the relative density is determined as D20/4 = 1.0233

Key value for chemical safety assessment

Relative density at 20C:

1.023

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method, is available. It is considered as a key study, and the result is retained as key data.