Registration Dossier
Registration Dossier
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EC number: 944-856-1 | CAS number: -
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 = 615 mg/l based on frond number
Key value for chemical safety assessment
Additional information
No studies on the "toxicity to aquatic plants other than algae" are available for the substance in itself nevertheless a study have been conducted with an analogue molecule (Similar Substance 03). Further information are reported in the Read Across justification attached to section 13.
The inhibitory effects of the test substance the duckweed Lemna minor were investigated over a period of 7 days, based on the frond number and biomass (dry weight), following the guideline OECD 221.
The test solutions were prepared by respective dilutions of a stock solution in Swedish standard-Medium (SIS Medium).
The test was performed at 611, 193, 61.1, 19.3 and 6.11 mg/l nominal concentration of the test item, corresponding to 500, 158.1, 50.0, 15.8 and 5.00 mg/l of the active ingredient.
Three parallel test vessels were used for each test concentration of the test item and six vessels for the blank controls.
The test concentrations during the 7-day test period were determined by photometry at the beginning of the test, as well as after 3, 5 and 7 days of exposure. These analyses confirmed the right dosage of the test item, and showed that the concentrations of the loadings decreased over the whole 7-day test period after 7 days and did not remain within 80-120 % range of the initial value (67, 69, 103, 90 and 86 %, respectively, of the initial value). Therefore, the effective concentrations (ErC50and EyC50) were assessed based on the geometric mean (GM) of the measured concentrations of the active ingredient.
The two endpoints frond number and biomass (dry weight) were investigated at days 3, 5 and 7, and each of them were assessed as growth rate and yield. The EC50 values obtained were:
ErC50 = 615 mg/l based on frond number
EyC50 = 197 mg/l based on frond number
ErC50 = 889 mg/l based on dry weight
EyC50 = 178 mg/l based on dry weight
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