Registration Dossier

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Biodegradability of test chemical 1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate (CAS no.84000-82-8) was estimated by

using OECD QSAR tool box v3.3 by considering six closest read across chemicals with log Kow as primary descriptor. 1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate undergoes 0.92% biodegradation by considering BOD as parameter and microrganisms as inoculum in 28 days. On the basis of this percent biodegradability value it can be concluded that test chemical 1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate is not readily biodegradable.

Additional information

Biodegradation in water :

Various predicted data for the target compound1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate ( CAS no. 84000 -82 -8) and supporting weight of evidence studies for its read across substance were reviewed for the biodegradation end point which are summarized as below:

 

Biodegradability of test chemical 1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate (CAS no.84000-82-8) was estimated by using OECD QSAR tool box v3.3 by considering six closest read across chemicals with log Kow as primary descriptor. 1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate undergoes 0.92% biodegradation by considering BOD as parameter and microrganisms as inoculum in 28 days. On the basis of this percent biodegradability value it can be concluded that test chemical 1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate is not readily biodegradable.

In another prediction by Estimation Programs Interface Suite (EPI suite, 2017) was run to predict the biodegradation potential of the test compound   1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate(CAS no.84000 -82 -8) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical  1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate is expected to be not readily biodegradable.

The above estimated results were supported by experimental result summarized in Advisory Board for Environmentally Relevant Existing Substances (BUA), Vol. 221, Pg. no. 42, 2000 for structurally and functionally read across substance N-benzyl-N-ethylaniline (CAS: 92-59-1).

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance N-benzyl-N-ethylaniline (CAS no.92-59-1).The percentage degradation of test substance N-benzyl-N-ethylaniline was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, N-benzyl-N-ethylaniline is considered to be not readily biodegradable in nature.

The above experimental data was further supported by experimental result summarized in Japan chemicals collaborative knowledge database (J-check), 2017 for structurally and functionally similar read across substance N, N-dimethylaniline (121-69-7).

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance N, N-dimethylaniline (CAS no. 121-69-7). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance N, N-dimethylaniline was determined to be1.9, 12.3 and 0% by BOD, TOC removal and GC parameter in 14 days. Thus, based on percentage degradation, N, N-dimethylaniline is considered to be not readily biodegradable in nature.

On the basis of above results for target chemical1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetate(from OECD QSAR toolbox version 3.3 and EPI suite, 2017) and for its read across substance (from authoritative database J-CHECK &Advisory Board for Environmentally Relevant Existing Substancesand peer reviewed journal), it can be concluded that the test substance1,4-dimethyl-5-[[4-[methylbenzylamino]phenyl]azo]-1H-1,2,4-triazolium acetatecan be expected to be not readily biodegradable in nature.