Registration Dossier

Administrative data

Endpoint:
density, other
Remarks:
density
Type of information:
other: Category approach
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Density data have been generated for various protein families. The data published in the cited book comes from 20 different protein families and they all have densities in the range from 1.32 g/ml to 1.42 g/ml. The density for arylesterase has not been determined, but since it is a protein it is expected, with very high reliability, to also have a density in this range.

Data source

Reference
Reference Type:
review article or handbook
Title:
Proteins: structures and molecular properties
Author:
Thomas E. Creighton
Year:
1993
Bibliographic source:
W.H.Freeman and Company; ISBN 0-7167-2317-4

Materials and methods

Principles of method if other than guideline:
Determination of the sedimentation constant allows calculation of the density using the Svedberg equation. This has been performed in peer reviewed articles and summarised in Creighton "Proteins: structure and molecular properties" (1993).
GLP compliance:
not specified
Type of method:
other: See "Principles of method"

Test material

Constituent 1
Reference substance name:
Protein as a constituent of enzyme deriving from the fermentation or extraction process
Molecular formula:
Not available. See remarks.
IUPAC Name:
Protein as a constituent of enzyme deriving from the fermentation or extraction process
Details on test material:
20 different proteins from different families have been analysed in peer reviewed articles and summarised in Creighton "Proteins: structure and molecular properties" (1993) .

Results and discussion

Density
Key result
Type:
density
Density:
> 1.33 - < 1.42 g/cm³
Temp.:
20 °C
Remarks on result:
other: density range for 20 different proteins from different protein-families

Any other information on results incl. tables

Creighton reviews density data retrieved from structurally and fuctionally different proteins. They all have densities in the rangebetween1.32 and 1.42 g/ml.

We ascertain that arylesterase also has a density in this range since it is a protein, even though not experimentally determined.

Applicant's summary and conclusion

Conclusions:
Arylesterase is a protein and therefore it can be assumed that this has density within the range 1.32 - 1.42 g/ml.
Executive summary:

Densities have been determined for a wide range of different proteins. This analysis showed that the density range is between 1.32 and 1.42 g/ml. Since arylesterase is a protein, we conclude that this protein also has density within this range.