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EC number: 280-744-2 | CAS number: 83763-57-9
In a stepwise procedure, increasing volumes of demineralized water were added at room temperature to a defined amount of the test item. After each addition of an amount of water the mixture was treated with ultrasound for 15 minutes and visually checked for any undissolved particles.
Table3: Results of the preliminary test
Amount of test item
Added volume of demineralized water
Total volume of demineralized water
Appearance of mixture
cloudy, not dissolved
slightly cloudy, dissolved
The preliminary test showed that the water solubility of the test item is > 10 mg/L. According to the preliminary test the water solubility was determined by the flask method in the main test. As the water solubility determined in the preliminary test was very high, an additional stirring test was performed to determine the highest amount of test item that can still be stirred sufficiently in a mixture with water.
Table4: Results of the stirring test
Added volume of demin. water
could be stirred
According to the stirring test mixtures with a concentration of approx.560 g/L test item in demin. water were used in the main test.
The flask method was performed as described above. The data for the evaluation are given in Table5. After the equilibration time precipitate was observed in the flasks of the experiments excluding in the blank experiment.The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given in Table5.
Table5: Water solubility experiments of the test item
24 h experiment
48 h experiment
72 h experiment
experiment (72 h)
Amount of test item / g
Amount of demineralized water / mL
Stirring time at 30 °C / h
pH at 24 °C
Measured concentration / mg/L
3.741 / 3.763
3.775 / 3.781
3.559 / 3.565
≤ 0.013* / ≤ 0.013*
Mean concentration / mg/L
Water solubility / g/L
* Quantification limit for measurement (lowest calibration standard)
The water solubility of the test item at 20 °C was determined to be1.85 g/L (± 0.06 g/L).
The water solubility cS of the test item Reactive Orange 64 at a temperature of 20 °C was determined according to the flask method. It was found to be:
cS= 1.85 g/L (± 0.06 g/L)
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