1-bromoheptadecafluorooctane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 January 2017 - 28 January 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

temperature range in excess of ± 1 ºC This was considered to have had no adverse effect on the integrity of the test, no adverse effects to exposure were observed in any of the control or test vessel.

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-bromoheptadecafluorooctane

EC Number:

207-028-4

EC Name:

1-bromoheptadecafluorooctane

Cas Number:

423-55-2

Molecular formula:

C8BrF17

IUPAC Name:

1-bromoheptadecafluorooctane

Test material form:

liquid

Specific details on test material used for the study:

Physical state/Appearance: Clear colorless liquidBatch: PFOBV42001Purity: 99.8%Expiry Date: 01 January 2018Storage Conditions: Room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken from the solvent control and the 0.020 mg/L test group from the bulk test preparation at 0 hours and from the pooled replicates at 48 hours for quantitative analysis.

Test solutions

Vehicle:

yes

Remarks:

dimethylformamide

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: A nominal amount of test item (20 mg) was dissolved in a final volume of 10 mL of dimethylformamide to give a 20 mg/10 mL solvent stock solution from which a dilution was made to give a further solvent stock solution of 2.0 mg/10 mL. An aliquot (100 μL) of the 2.0 mg/10 mL solvent stock solution was dispersed in 1 liter of test media with the aid of magnetic stirring for approximately 10 minutes to give the required test concentration of 0.020 mg/L and a final solvent concentration of 100 μL/L. The control consisted of test medium only and the solvent control consisted of test medium and dimethylformamide at a final solvent concentration of 100 μL/L.- Controls: control (Elendt M7) and solvent control- Chemical name of vehicle (organic solvent, emulsifier or dispersant):dimethylformamide- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):100 μL/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM - Source: in-house laboratory cultures - Feeding during test: none ACCLIMATIONCultures maintained under test conditions, free from disease METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing.

Study design

Test type:

static

Water media type:

other: Elendt M7 Medium

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

18-20 deg C

pH:

7.6 - 8.0

Dissolved oxygen:

7.6 -9.7 mg/L

Nominal and measured concentrations:

Nominal 0.020 mg/L; measured 0 hours 0.013 mg/L; 48 hours 0.0096 mg/L. Geometric mean measured = 0.011 mg/L.solvent control at 0 and 48 hours

Details on test conditions:

TEST SYSTEM - Test vessel: Conical flasks - Type (delete if not applicable): closed, sealed with a ground glass stopper - Material, size, headspace, fill volume: glass, 100 mL completely filled - Aeration: none - Type of flow-through (e.g. peristaltic or proportional diluter): static - No. of organisms per vessel: 5 - No. of vessels per concentration (replicates): 4 - No. of vessels per control (replicates): 4 - No. of vessels per vehicle control (replicates): 4 TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: Elendt M7 medium prepared as described in the OECD Test guideline OTHER TEST CONDITIONS - Adjustment of pH: none - Photoperiod:16 hours light and 8 hours dark with 20 minute transition periods. - Light intensity: 200 - 1200 Lux EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Any immobilization or adverse reactions to exposure were recorded at 24 and 48 hours after the start of exposure. The criterion of effect used was that daphnia were considered to be immobilized if they were unable to swim within 15 seconds after gentle agitation. VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 0.011 mg/L

Nominal / measured:

meas. (geom. mean)

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.011 mg/L

Nominal / measured:

meas. (geom. mean)

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.011 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: - Observations on body length and weight: none - Other biological observations: A single immobilized daphnid was observed to be immobilized in the 0.011 mg/L test preparation after 48 hours exposure. This was considered to be due to natural causes rather than a true toxic effect given that only 5% immobilization occurred. - Mortality of control: No immobilization was observed in the control and solvent control vessels - Other adverse effects control: No sub-lethal effects of exposure were observed throughout the test.

Results with reference substance (positive control):

- Results with reference substance valid: yes- Relevant effect levels: immobilisation 24 hour EC50- Dose-response test: 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L- ECx: 24 hour EC50 = 1.2 mg/L within the normal range for this reference item (The results of the inter laboratory tests and a Technical Corrigendum to ISO 6341 give an EC50-24 hour for potassium dichromate within the range 0.6 mg/L to 2.1 mg/L.)

Reported statistics and error estimates:

An estimate of the EC50 values was given by inspection of the immobilization data.The No Observed Effect Concentration (NOEC) was determined by inspection of the immobilization data, with less than 10% immobilization being considered insignificant.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and, based on the geometric mean measured test concentration gave a 48-Hour EC50 value of greater than 0.011 mg/L. The No Observed Effect Concentration was 0.011 mg/L.This study showed that there were no toxic effects at the limit of solubility.