Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

1
Chemical structure
Reference substance name:
Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
EC Number:
226-408-0
EC Name:
Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
Cas Number:
5395-50-6
Molecular formula:
C8H14N4O6
IUPAC Name:
1,3,4,6-tetrakis(hydroxymethyl)-octahydro-[1,3]diazolo[4,5-d]imidazole-2,5-dione

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Origin
Institut für Wasser-, Boden- und Lufthygiene des Bundesgesundheitsamtes,
Corrensplatz, D-14195 Berlin

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
244 mg/L CaCO3
Test temperature:
20.4
pH:
7.67
Dissolved oxygen:
8.93 mg/L
Conductivity:
657 µS/cm
Nominal and measured concentrations:
Nominal: 6.25 - 100 mg/L (5 concentration leveis in a geometrical series with a factor of 2)
The concentrations of the active ingredient Tetramethylolacetylene diurea (4-ADH) were calcuIated
based on the results of the formaidehyde (HCHO) determinations. The recovery rates were in the
range 28 - 84 %.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 38.9 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
4-ADH
Basis for effect:
mobility
Results with reference substance (positive control):
A reference test is carried out once per month with potassium dichromate as reference item and the
reference toxicity determined. The EC50-value of the reference item at 1.9 mg/IL after 24 h was within
the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30.
Water quality parameters of pH-value, dissolved oxygen concentration and temperature measured at
0 and 48 h were determined to be within the acceptable limits.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes