Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-335-2 | CAS number: 7493-74-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 Apr - 17 May 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Niedersächsiches Umweltministerium, Hannover, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Sewage treatment plant Hildesheim, Germany
- Laboratory culture: no
- Pretreatment: Filtered with folded filter without using the first 200 mL of the filtrate. The second filtrate effluent from the domestic waste water is used to initiate inoculation.
- Colony forming units: 9.2E+07 CFU/L (inoculum); 6.1E+04 CFU/L (in test vessel) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 4 mg/L
- Based on:
- test mat.
- Initial conc.:
- 8.32 mg/L
- Based on:
- ThOD/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20.5 - 21.0 °C
- pH: 7.45 - 7.56 (begin), 7.25 - 7.64 (end)
- pH adjusted: no
- Aeration of dilution water: One day before medium preparation the dilution water was aerated until oxygen saturation and then left at room temperature for at least 20 h.
TEST SYSTEM
- Culturing apparatus: BOD bottles with glass stoppers, 300 mL test volume
- Number of culture flasks/concentration: 10
- Method used to create aerobic conditions: The dilution water was aerated until oxygen saturation.
- Measuring equipment: Oximeter, WTW Oxi 530
- Test performed in closed vessels: yes
- Other: Test solutions were made as stock solutions. 0.2 mL of the inoculum was given in each BOD bottle.
SAMPLING
- Sampling frequency: on day 0, 7, 14, 21 and 28
- Sampling method: Oxygen concentration was measured in the incubation vessels.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 10 bottles
- Toxicity control: yes, 10 bottles
- Other: Reference substance: yes, 10 bottles - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 28 d
- Details on results:
- The 10-day window was reached (10% degradation after 4 d and 69% after 14 d). Thus, the test substance can be regarded as readily biodegradable according to the OECD criteria. The toxicity control attained 42% after 14 d. Since more than 25% biodegradation occurred, the test substance is not inhibitory to the inoculum.
- Results with reference substance:
- 80% after 21 d, 60% pass level was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The tested substance is readily biodegradable (68% after 28 d) under tested conditions (OECD 301D) and the 10-day window criterion was met.
- Executive summary:
The ready biodegradability of the substance by domestic, non-adapted activated sludge was performed according to the OECD guideline 301 D and GLP. The test was run for 28 days and microorganisms were exposed to 4 mg/L test material corresponding to 8.32 mg/L ThOD/L. O2 consumption was followed for the estimation of the biodegradation. Acetic acid (10 mg/L) was used as a reference substance. A toxicity control was set up with 2 mg/L test item and 5 mg/L reference substance in order to examine the toxicity of the tested substance on microorganisms. 68% biodegradation took place after 28 d, the 10-day window criterion was fulfilled and therefore the tested substance is considered to be readily biodegradable. The toxicity control attained 42% degradation after 14 d, verifying that the tested item was not inhibitory to the inoculated microorganisms.
Reference
Table 1: Degradation of the test substance, reference substance and toxicity control.
Sampling day |
Test substance [%] |
Reference substance [%] |
Toxicity control [%] |
7 |
21 |
78 |
27 |
14 |
69 |
79 |
42 |
21 |
65 |
80 |
52 |
28 |
68 |
74 |
48 |
Description of key information
Readily biodegradable: 68% biodegradation after 28 d, 10-day window criterion met (OECD 301 D).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
One study is available, testing the ready biodegradability of the substance by domestic, non-adapted activated sludge. The test was performed according to the OECD guideline 301 D and GLP. The test was run for 28 days and microorganisms were exposed to 4 mg/L test material corresponding to 8.32 mg/L ThOD/L. O2 consumption was followed for the estimation of the biodegradation. Acetic acid (10 mg/L) was used as a reference substance. A toxicity control was set up with 2 mg/L test item and 5 mg/L reference substance in order to examine the toxicity of the tested substance on microorganisms. 68% biodegradation took place after 28 d, the 10-day window criterion was fulfilled and therefore the tested substance is considered to be readily biodegradable. The toxicity control attained 42% degradation after 14 d, verifying that the tested item was not inhibitory to the inoculated microorganisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.