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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
To produce the test solution, the test substance was weighed into water and treated in an ultrasonic bath for 1 hour. Additionally the test solution was stirred on a magnetic stirrer for 24 hours.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
-Species: Zebra fish (Brachydanio rerio HAMILTON BUCHANAN)
-Origin: Bio International B.V. (Netherlands)
-Date of birth: 16.01.1997
-Date supplied: 08.04.1997
-Body length: 2.5-3.5 cm
-Mortality while kept: < 2 %
-Medical pretreatment: none
-Keeping water: Synthetic fresh water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
n.a.
Test temperature:
21.9-23.3 °C
pH:
7.1-8.4
Dissolved oxygen:
4.3-8.1
Salinity:
n.a.
Conductivity:
n.a.
Nominal and measured concentrations:
nominal concentrations: 10.0, 31.6, 100 mg/L
Details on test conditions:
STUDY CONDITIONS:
-Operating conditions: static test
-Photoperiod: 16 hours light / 8 hours dark
-Test procdure:
-Test vessels (300 x 135 x 200 mm)
-5 L test medium
-ventilated
-static
-Method of administration: Direct weighing
-Test concentrations: 10, 31.6, 100 mg/L
-Solubilizer or other auxiliaries: Ultrasonic bath, magnetic stirrer
-Control: Synthetic fresh water
-Water for dilution: Synthetic fresh water

Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
24 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: geometric mean: LC0/LC100
Validity criteria fulfilled:
no
Remarks:
validity criteria are fulfilled, but there is no evidence that the concentration of the test substance has been satisfactorily maintained (at least 80 % of nominal concentration) as no analytical monitoring is reported
Conclusions:
The acute toxicity of the test item to fish (Danio rerio) was conducted in a static test according to EU Method C.1 (Acute Toxicity to Fish). After 96 hours of exposure a LC0 value of 10 mg/L and after 24 hours of exposure a LC100 value of 100 mg/L was obtained. A geometric mean of LC0/LC100 was calculated to be 31.6 mg/L.
Executive summary:

Acute toxicity of the test item to fish (Danio rerio) was conducted according to EU Method C.1 (Acute Toxicity to Fish) which is in most parts equivalent to OECD Guideline for Testing of Chemicals No. 203 (Fish, Acute Toxicity Test). Fish were exposed to the test item under static conditions for 96 hours to a range of concentration (10, 31.6, 100 mg/L). Mortality of fish was recorded at 24-hour intervals. Additionally any visible effects on appearance and behaviour of fish was reported every 24 hours. During the test a temperature range of 21.9 - 23.3 °C and a pH range of 7.1 - 8.4 was maintained in the test vessels. Auxiliaries used to prepare the test media were an ultrasonic bath and a magnetic stirrer. No specific analysis was performed. After 96 hours of exposure an LC0 value of 10 mg/L and after 24 hours an LC100 a value of 100 mg/L could be determined. A geometric mean of LC0/LC100 was calculated to be 31.6 mg/L. The results are expressed in terms of nominal concentrations. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute fish study.

Description of key information

The acute toxicity of the test item to fish (Danio rerio) was conducted in a static test according to EU Method C.1 (Acute Toxicity to Fish). After 96 hours of exposure a LC0 value of 10 mg/L and after 24 hours of exposure a LC100 value of 100 mg/L was obtained. A geometric mean of LC0/LC100 was calculated to be 31.6 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
31.6 mg/L

Additional information

Acute toxicity of the test item to fish (Danio rerio) was conducted according to EU Method C.1 (Acute Toxicity to Fish) which is in most parts equivalent to OECD Guideline for Testing of Chemicals No. 203 (Fish, Acute Toxicity Test). Fish were exposed to the test item under static conditions for 96 hours to a range of concentration (10, 31.6, 100 mg/L). Mortality of fish was recorded at 24-hour intervals. Additionally any visible effects on appearance and behaviour of fish was reported every 24 hours. During the test a temperature range of 21.9 - 23.3 °C and a pH range of 7.1 - 8.4 was maintained in the test vessels. Auxiliaries used to prepare the test media were an ultrasonic bath and a magnetic stirrer. No specific analysis was performed. After 96 hours of exposure an LC0 value of 10 mg/L and after 24 hours an LC100 a value of 100 mg/L could be determined. A geometric mean of LC0/LC100 was calculated to be 31.6 mg/L. The results are expressed in terms of nominal concentrations. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute fish study.