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EC number: 259-709-0 | CAS number: 55566-30-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 1989 to April 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Similar to guideline study, with no major deficiencies. No compounds other than 2-aminoanthracene were used as positive control compounds in the presence of S9. No tests were carried out with E.coli WP2 uvrA or S. typhimurium TA 102. The test substance stability was not tested but this is considered not to have adversely affected the study results since dosing solutions were daily prepared freshly before testing.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- : 2-aminoanthracene was the only positive control compound tested with metabolic activation. No test carried out with E.coli WP2 uvrA or S. typhimurium TA 102. Test substance stability was not tested.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tetrakis(hydroxymethyl)phosphonium sulphate(2:1)
- EC Number:
- 259-709-0
- EC Name:
- Tetrakis(hydroxymethyl)phosphonium sulphate(2:1)
- Cas Number:
- 55566-30-8
- Molecular formula:
- C4H12O4P.1/2O4S
- IUPAC Name:
- tetrakis(hydroxymethyl)phosphonium sulphate(2:1)
- Test material form:
- other: liquid stored in the dark at ambient temperature
- Details on test material:
- - Name of test material (as cited in study report): THPS-75
- Physical state: colourless liquid
- Stability under test conditions: not tested
- Storage condition of test material: stored in the dark at ambient temperature
Constituent 1
Method
- Target gene:
- Three mutations in the histidine operon are involved:
- his G 46 in TA1535 and TA100
- his C 3076 in TA1537
- his D 3052 in TA1538 and TA98
All 5 strains contained the deep rough; rfa and uvrB mutations. These 2 deletions included the nitrate reductase (chl) and biotin (bio) genes also.
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium strains TA1535, TA 1537, TA 1538, TA98, TA100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix prepared from liver of male Fischer 344 rats induced with Arochlor 1254.
- Test concentrations with justification for top dose:
- For both experiments, both with and without S9 mix:
Expressed as active substance: 0, 1, 3, 10, 33, 100, 333 µg/plate,
Expressed as main ingredient: 0, 0.75, 2.3, 7.5, 24.9, 75.5, 251.4 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: sterile ultra pure water
- Justification for choice of solvent/vehicle: no data
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- sterile ultra pure water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2-AAN)
- Remarks:
- with S9 for all bacteria strains
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without S9 for TA 1535 and TA 100
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S9 for TA 1537
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- without S9 for TA 1538 and TA 98
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: none
- Incubation period: at 37° for 48 hours.
NUMBER OF REPLICATIONS: 3 plates/dose/strain
DETERMINATION OF CYTOTOXICITY
- Method: background lawn of microcolonies observation - Evaluation criteria:
- Acceptance criteria:
A test was considered acceptable if for each strain:
- the bacteria demonstrated their typical responses to crystal violet, ampicillin and u.v. light
- at least 2 of the vehicle control plates were within the following colony ranges number:
TA1535: 4 -30
TA1537: 1 -20
TA98: 10 -60
TA100: 60 -200 and
TA1538: 5 -35
- on a least 2 of the positive control plates, there were x 2 the mean of vehicle control mutant numbers per plate, or in case of TA100; x 1.5
- no toxicity or contamination was observed in at least 4 dose levels
- in case where a mutagenic response was observed, that no more than one dose level was discarded before the dose which gave the highest significant mean colony number.
A mutagenic response was recorded if:
- the test substance induced a doubling in the number of revertants when compared to that in the vehicle controls, at some concentrations. (for TA100, a 1.5 fold increase over the control value is required)
- a significant dose relationship is shown, although at high dose levels, this relationship could be inverted because of, for example toxicity to bacteria generally, specific toxicity to the mutants or inhibition of foreign compound metabolising enzymes where mutagens require metabolic activation by the liver.
- a reproducible effect in independent tests is demonstrated. - Statistics:
- no data
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 251.4 µg/plate and above
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS:
- Precipitation: There was no precipitation of the test compound
RANGE-FINDING/SCREENING STUDIES: a preliminary toxicity study was carried out in TA100 only at concentrations of 24.9 to 7550 µg main ingredient/plate (33 to 10000 µg active substance/plate) with and without S9. Complete killing of the bacteria (ie no background lawn of micro-colonies) occurred at 1000 µg, 3333 µg per plate. Toxicity to the bacteria resulting in a thinning of the background lawn of microcolonies was observed at 333 µg per plate in both the presence and the absence of S9 mix.
COMPARISON WITH HISTORICAL CONTROL DATA: Yes - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 8.5.1. Gene Mutation Assay in the absence of S9 mix
Concentration [µg/plate as main ingredient] |
Number of mutant cells |
|||||||||
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
||||||
Test I |
Test II |
Test I |
Test II |
Test I |
Test II |
Test I |
Test II |
Test I |
Test II |
|
0 |
5 |
6 |
9 |
3 |
7 |
12 |
14 |
15 |
112 |
78 |
0.75 |
4 |
5 |
10 |
4 |
7 |
13 |
14 |
15 |
94 |
86 |
2.3 |
5 |
4 |
7 |
5 |
6 |
8 |
15 |
17 |
114 |
75 |
7.5 |
5 |
5 |
11 |
4 |
7 |
8 |
13 |
13 |
95 |
67 |
24.9 |
5 |
1 |
9 |
3 |
9 |
9 |
14 |
10 |
99 |
80 |
75.5 |
4 |
6 |
7 |
9 |
6 |
11 |
12 |
6 |
118 |
93 |
251.4 |
3* |
1* |
2* |
6* |
2* |
0* |
0* |
0* |
49* |
26* |
+ve control |
54 |
40 |
111 |
135 |
177 |
167 |
161 |
100 |
233 |
185 |
* Observation of lawn microcolonies due to compound cytotoxicity
Table 8.5.2. Gene Mutation Assay in the presence of S9 mix
Concentration [µg/plate as main ingredient] |
Number of mutant cells |
|||||||||
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
||||||
Test I |
Test II |
Test I |
Test II |
Test I |
Test II |
Test I |
Test II |
Test I |
Test II |
|
0 |
9 |
5 |
8 |
5 |
8 |
18 |
14 |
13 |
101 |
106 |
0.75 |
12 |
4 |
11 |
3 |
14 |
19 |
16 |
9 |
110 |
108 |
2.3 |
8 |
3 |
8 |
1 |
18 |
17 |
11 |
7 |
113 |
85 |
7.5 |
10 |
4 |
6 |
2 |
10 |
20 |
16 |
7 |
121 |
89 |
24.9 |
8 |
3 |
7 |
6 |
16 |
9 |
13 |
7 |
98 |
77 |
75.5 |
13 |
7 |
9 |
4 |
15 |
33 |
14 |
10 |
102 |
87 |
251.4 |
5* |
1* |
3* |
1* |
7* |
8* |
9* |
3* |
46* |
9* |
+ve control |
67 |
34 |
79 |
44 |
355 |
209 |
444 |
146 |
802 |
293 |
* Observation of lawn microcolonies due to compound cytotoxicity
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this study, THPS had no mutagenic activity on S. typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100, with and without metabolic activation.
- Executive summary:
Tolcide PS75 (75.5% THPS main ingredient) was tested for mutagenic activity in S. typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 according to the method of Ames et al. (1975), similar to the OECD Guidelines 471.
A preliminary toxicity study was carried out in TA100 only at concentrations of 33 to 10000µg active substance/plate with and without S9, corresponding to 24.9 to 7550 µg main ingredient/plate.
The mutagenicity tests were conducted on agar plates at concentrations ranging from 0.75 to 251.4 µg main ingredient/plate with and without S9 mix from Arochlor 1254 treated rat liver.
Toxicity was seen as thinning of the bacterial lawn at 251.4 µg/plate with and without S9 and total lack of lawn above this concentration. There was no precipitation of the test substance.
The test substance did not induce mutagenic activity in either the presence or absence of S9 mix in any of the 5 bacterial strains used. The positive controls induced the appropriate responses in the corresponding strains.
In conclusion, THPS had no mutagenic activity in the applied bacterium tester strains under the test conditions used in this study.
This study is considered as acceptable as it satisfied the criteria of the OECD guideline N°471.
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