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Long-term toxicity to fish

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Reference
Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Long term toxicity to Heteropneustes fossilis (Catfish) study was carried out for 60 days.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): (4-(4-aminophenyl)(4-iminocyclohexa-2,5-dienylidene)methyl)-2-methylaniline hydrochloride
- Common name: Fuschin basic
- Molecular formula: C20H19N3.HCl
- Molecular weight: 337.852
- Smiles notation: C(\c1cc(c(N)cc1)C)(c1ccc(N)cc1)=C1/C=CC(=N)C=C1.Cl
- InChl: 1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,21H,22-23H2,1H3;1H/b20-14-,21-17?;
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: Test chemical concentration used for the study were 6.24 mg/l (for 96 h), 3.12 mg/l (for 10-20 days) and 1.56 mg/l (for 10 days), respectively.
Vehicle:
not specified
Test organisms (species):
other: Heteropneustes fossilis
Details on test organisms:
TEST ORGANISM
- Common name: Catfish
Test type:
not specified
Water media type:
brackish water
Total exposure duration:
60 d
Reference substance (positive control):
not specified
Key result
Duration:
60 d
Dose descriptor:
NOEC
Effect conc.:
3.12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: No significant difference in the serum calcium and protein levels was observed from that of controls.
Details on results:
Exposure of the catfish to acute (6.24 ppm) level of fuchsin basic for 96 h and to sub-acute (3.12 ppm) for short (10-20 days) and sub-lethal (1.56 ppm) for 10 days, resulted in significant decrease in serum calcium and protein levels. However, long term (30-60 days) exposure of test organism to both 3.12 mg/l and 1.56 mg/l levels, did not produced significant difference in the serum calcium and protein levels from that of controls.
Validity criteria fulfilled:
not specified
Conclusions:
For chemical, (4-(4-aminophenyl)(4-iminocyclohexa-2,5-dienylidene)methyl)-2-methylaniline hydrochloride, as no significant difference in the serum calcium and protein levels was observed from that of controls, the 60 days NOEC value was determined to be 3.12 mg/l, respectively.
Executive summary:

Long term toxicity to Heteropneustes fossilis (Catfish) study was carried out for 60 days.The study was based on the effects of the test compound (4-(4-aminophenyl)(4-iminocyclohexa -2,5 -dienylidene)methyl)-2-methylaniline hydrochloride (CAS no. 632-99-5) on Heteropneustes fossilis in a brackish water system. Heteropneustes fossilis (Catfish) was used as a test organism. Test chemical concentration used for the study were 6.24 mg/l (for 96 h), 3.12 mg/l (for 10-20 days) and 1.56 mg/l (for 10 days), respectively. As no significant differences in the serum calcium and protein levels was observed from that of controls, the 60 days NOEC value was determined to be 3.12 mg/l, respectively.

Description of key information

Long term toxicity to Heteropneustes fossilis (Catfish) study was carried out for 60 days(American chemical society, 2017). The study was based on the effects of the test compound (4-(4-aminophenyl)(4-iminocyclohexa -2,5 -dienylidene)methyl)-2-methylaniline hydrochloride (CAS no. 632-99-5) on Heteropneustes fossilis in a brackish water system. Heteropneustes fossilis (Catfish) was used as a test organism. Test chemical concentration used for the study were 6.24 mg/l (for 96 h), 3.12 mg/l (for 10-20 days) and 1.56 mg/l (for 10 days), respectively. As no significant differences in the serum calcium and protein levels was observed from that of controls, the 60 days NOEC value was determined to be 3.12 mg/l, respectively.

Key value for chemical safety assessment

Additional information

Long term toxicity to Heteropneustes fossilis (Catfish) study was carried out for 60 days(American chemical society, 2017). The study was based on the effects of the test compound (4-(4-aminophenyl)(4-iminocyclohexa -2,5 -dienylidene)methyl)-2-methylaniline hydrochloride (CAS no. 632-99-5) on Heteropneustes fossilis in a brackish water system. Heteropneustes fossilis (Catfish) was used as a test organism. Test chemical concentration used for the study were 6.24 mg/l (for 96 h), 3.12 mg/l (for 10-20 days) and 1.56 mg/l (for 10 days), respectively. As no significant differences in the serum calcium and protein levels was observed from that of controls, the 60 days NOEC value was determined to be 3.12 mg/l, respectively.