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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
The test was performed with M4 medium according to OECD 202 (2004). The test was performed in the static version without change of test solutions.
Test organisms (species):
Daphnia magna
Details on test organisms:
The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and was cultivated at Hydrotox GmbH since October 2012. The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into fresh M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C ×daphnid-1×day-1.
The test organisms used were 3.75 – 20.75 h old at the start of the test. Quality assurance takes place at regular intervals by testing the sensitivity of the test organisms to the reference item potassium dichromate (Sigma, Steinheim, Germany; Lot no.: MKBF2111V, expiration date: February 2018). The recent quality testing was performed in October 2017 with EC50 = 1.77 mg/l (1,63 – 2,05 mg/L Cl-95%) (24 h) which is in the declared range of 0.6 - 2.1 mg/L (24 h) according to OECD 202.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
Daphnids, less than 24 h old, were exposed to the test item in M4 medium for 48 h. The swimming capability of the daphnids was assessed after 24 h and 48 h exposure.
Post exposure observation period:
48 h
Hardness:
The test was performed with M4 medium according to OECD 202 (2004).
Test temperature:
The temperature during the exposure was 20.1 – 21.0 °C (required 18 - 22 °C, constant within ± 1 °C).
pH:
The pH was 7.4 - 7.6 in the control and 7.6 – 8.2 in the test item treatment.
Dissolved oxygen:
The dissolved oxygen concentration was 7.8 – 8.0 mg/L in the control and 7.8 – 8.1 mg/L in the test item treatment.
Salinity:
The test was performed with M4 medium according to OECD 202 (2004).
Conductivity:
The test was performed with M4 medium according to OECD 202 (2004).
Details on test conditions:
M4 medium; the test was performed in the static
version without change of test solutions.

The test vessels were kept in an incubator with 16 h light : 8 h dark for 48 h.
The swimming capability of the daphnids was determined after 24 h and 48 h exposure. Animals that did not swim within 15 s after gently moving were considered to be immobile. The pH as well as the O2-concentrations were measured at the start (0 h) and at the end of the test (48 h)
The temperature was measured continuously.
Reference substance (positive control):
no
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
> 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
> 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
> 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 10.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
No immobility could be observed.
Executive summary:

The Daphnia magna acute immobilisation test was performed as a limit test. The measured TOC-concentration of the test item treatment was 84.3% (0h) and 100.8% (48h) of the nominal

test item concentration. Therefore the EC values are given as nominal concentrations. EC10 and EC50 after 24 and 48 hours is > 10.1 mg/l

No immobility could be observed.

Description of key information

No immobility could be observed.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
10.1 mg/L

Additional information

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