2,2,3,3,3-pentafluoropropanol

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

15 Jun - 12 Nov 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Non-GLP, the study was conducted according to the old test guidance. The basic data are given, but not the detailed BCF relevant determinations according to the current standard, e.g.no study of steady state, no lipid corrections, etc.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Circular on Test Methods of New Chemical Substances (Japan), Bioaccumulation test in fish

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

Remarks:

Comply with the standard at the time of testing

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2,3,3,3-pentafluoropropanol

EC Number:

207-012-7

EC Name:

2,2,3,3,3-pentafluoropropanol

Cas Number:

422-05-9

Molecular formula:

C3H3F5O

IUPAC Name:

2,2,3,3,3-Pentafluoropropan-1-ol

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Daikin Industries, Ltd.
- Expiration date of the lot/batch: no data
- Purity test date: 99.4%

Sponsors's data on the test material:
- State: clourless liquid
- BP: 80.3°C
- Density: 1.54 (25°C)
- Solubility in Water: > 2000 ppm

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: Took two fish of each test concentration for analtic at Week 2, 4, 6 and 8
- Sampling intervals/frequency for test medium samples: Took 25 mL test medium for concentrations determination at Day 2, 5, 10, 12, 16, 19, 23, 26, 30, 33, 38, 40, 44, 47, 51 and 54
- Sample storage conditions before analysis: no storage
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
- 2 fish were sampled during the uptake phase. After measurement of weight and length, fish was freeze-dried, minced, dissolved in dechlorinated water, urea added, distillated and dissolved in diethyl ether. Sodium chloride was added in diethyl ether layer. After repeated washing with diethyl ether, diethyl ether layer was filtered for dewatering for GC-MS sample.
- After sampling of the test water, sodium chloride was added. After washing with diethyl ether, the diethyl ether layer was filtered for dewatering and concentrated for GC-MS sample.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Length at study initiation (length definition, mean, range and SD): average length 10,2 cm
- Weight at study initiation (mean and range, SD): average length weiight 30,6 g
- Lipid content at test initiation (mean and range, SD): average lipid content 4.1%

Maitain:
- External disinfection: pretreament in 10 ppm HCl for 24 hours

ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions (same as test or not): 25°C

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Total depuration duration:

0 d

Test conditions

Hardness:

no data

Test temperature:

25 ± 1 °C

pH:

no data

Dissolved oxygen:

no data

TOC:

no data

Salinity:

no data

Conductivity:

no data

Details on test conditions:

TEST SYSTEM
- Test vessel: Test vessel for bioaccumulation test of volatile chemical substances
- Material, size, headspace, fill volume: glass, 100 L
- Type of flow-through (e.g. peristaltic or proportional diluter): propotional diluter: 2 mL/min (stock solution) : 800 mL/min (dilution water)
- Renewal rate of test solution (frequency/flow rate): 1125 L/d
- No. of organisms per vessel: 8 in each concentration
- No. of vessels per concentration (replicates): 1


RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: According to the TLm test, 240, 320, 420, 560, 750 and 1000 ppm was tested in Medaka fish.
- Results used to determine the conditions for the definitive study: TLm value for 48 h: 450 ppm (W/V)

Nominal and measured concentrations:

Nominal concentration: 1 and 0.1 ppm (W/V)
Mesured concentration: 868 ppb, 876 ppb, 864 ppb and 856 ppb, and 79.3, 91.2, 86,7. and 86.0 ppb at week 2, 4, 6 and 8, respectively

Reference substance (positive control):

no

Results and discussion

Depuration

Elimination:

no

Remarks on result:

not measured/tested

Details on results:

- Mortality of test organisms: no mortality
- Behavioural abnormalities: no adverse effects were observed

Any other information on results incl. tables

Table 1. Accumulation ratio of the test substance

Nominal concentration	2 W	4 W	6 W	8 W
1 ppm	(0.9)* (0.8)	<= 0.2 (0.8)	(0.4) (0.5)	(0.7) (0.7)
0.1 ppm	<= 2.3 <= 2.3	<= 2.3 <= 2.3	<= 2.3 <= 2.3	<= 2.3 <= 2.3

( )*: as reference value (see Table 2)

 

Table 2. The value range with reliable values, range for reference data, and range under detection limit

	Fish concentration	Accumulated ratio
Range with reliable value	<= 4.9	(1 ppm) >= 5.7 (0.1 ppm) >= 5.7
Range for reference values	0.2 – 4.9	(1 ppm) 0.2 - 5.7 (0.1 ppm) 2.3 – 5.7
Under detection limit	<= 0.2	(1 ppm) <= 0.2 (0.1 ppm) <= 2.3