Trisodium hydrogen [2-[[α-[[3-[[4-chloro-6-[ethyl[3-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulphophenyl]azo]benzyl]azo]-4-sulphobenzoato(6-)]cuprate(4-)

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two hydrolysis studies as-
WoE 2. and WoE 3.

GLP compliance:

not specified

Radiolabelling:

not specified

Analytical monitoring:

yes

Remarks:

WoE 2: yes and WoE 3: no data available

pH:

4

Temp.:

25 °C

Remarks:

WoE 2: The study was performed at different pH i.e, 4, 7 and 9 and at 25°C & 50⁰C

Remarks:

WoE 3: Details not known

Positive controls:

not specified

Negative controls:

not specified

Transformation products:

no

Remarks:

WoE 2: no and WoE 3: no data available

Temp.:

25 °C

DT50:

> 1 yr

Remarks on result:

other: WoE 2: The study was performed at different pH i.e, 4, 7 and 9

Remarks on result:

other: WoE 3: Test chemical was reported to be stable in water.

Details on results:

WoE 2: Test chemical does not undergo hydrolysis at pH 4, 7 and 9, respectively at 50⁰C for 5 days and thus was reported to be stable with a half-life of > 1 yr at 25°C at pH 4 to 9.
WoE 3: Test chemical was reported to be stable in water

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach, the half-life of test chemical can be expected to be > 1 year at 25°C. Thus, test chemical was considered to be stable in water.

Executive summary:

Data available of the read across chemicals has been reviewed to determine the hydrolysis half-life value of the test chemical. The studies are as mentioned below:

The half-life of the test chemical was determined at different pH range. The study was performed following the OECD Guideline 111 (Hydrolysis as a Function of pH) and other test guideline Directive 84/449/EEC, C.10 "Abiotic degradation: hydrolysis as a function of pH" guideline. The determination of test chemical in the samples was performed by high performance liquid chromatography (HPLC) using an external standard. For the confirmation of sterility, the buffered aqueous solutions were heated at 121 °C for 15 minutes before use. Exclusion of photolytic effects was accomplished using the brown glass reaction flasks. No degradation of the test chemical was observed at 50⁰C after 5 days. Thus, the half-life of test chemical was determined to be > 1 yr at 25°C at pH 4 to 9 and was reported to be stable.

For the test chemical, the half-life of the test chemical was determined. On the basis of chemical structure of test chemical, it do not undergoe hydrolysis under environmental temperature and pH. Thus, the test chemical was reported to be stable.

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach, the half-life of test chemical can be expected to be > 1 year at 25°C. Thus, test chemical was considered to be stable in water.

Description of key information

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach, the half-life of test chemical can be expected to be > 1 year at 25°C. Thus, test chemical was considered to be stable in water.

Additional information

Data available of the read across chemicals has been reviewed to determine the hydrolysis half-life value of the test chemical. The studies are as mentioned below:

The half-life of the test chemical was determined at different pH range. The study was performed following the OECD Guideline 111 (Hydrolysis as a Function of pH) and other test guideline Directive 84/449/EEC, C.10 "Abiotic degradation: hydrolysis as a function of pH" guideline. The determination of test chemical in the samples was performed by high performance liquid chromatography (HPLC) using an external standard. For the confirmation of sterility, the buffered aqueous solutions were heated at 121 °C for 15 minutes before use. Exclusion of photolytic effects was accomplished using the brown glass reaction flasks. No degradation of the test chemical was observed at 50⁰C after 5 days. Thus, the half-life of test chemical was determined to be > 1 yr at 25°C at pH 4 to 9 and was reported to be stable.

 

For the test chemical, the half-life of the test chemical was determined. On the basis of chemical structure of test chemical, it do not undergoe hydrolysis under environmental temperature and pH. Thus, the test chemical was reported to be stable.

 

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach, the half-life of test chemical can be expected to be > 1 year at 25°C. Thus, test chemical was considered to be stable in water.