1,1'-[methylenebis(sulphonyl)]diethylene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

29/12/1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Determination of the water biodegradability of organic chemicals by means of the two-phase air-water closed bottle test. NEN 6515

Version / remarks:

1992

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: Draft International Standard. Two phase closed bottle test. ISO/TC 147/SC5/WG4 N174

Version / remarks:

1993

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-[methylenebis(sulphonyl)]diethylene

EC Number:

221-909-0

EC Name:

1,1'-[methylenebis(sulphonyl)]diethylene

Cas Number:

3278-22-6

Molecular formula:

C5H8O4S2

IUPAC Name:

(ethenesulfonyl)methanesulfonylethene

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Mixed liquor is obtained from the aeration tank of the waste water treatment plant of Bocholt. This treatment plant is dealing predominantly with domestic sewage. The activated sludge is sampled on 18/10/93 and used on the same day. Before use, the mixed liquor is sieved through a 0.5mm sieve in order to remove large particles, the suspended solids content is determined (4.36 g/L) and the mixed liquor is washed with test medium. Afer settlement of the sludge, the liquid phase is discarded and the solids are resuspended in test medium to obtain a concentration of 3 g/L (= inoculum suspension).
A dilution of 10 ml of the inoculum suspension in 1 litre test medium is made to obtain a suspended solids concentration of 30 mg/l (= inoculated test medium).

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

15.4 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 20-25°C
- pH: 7.5
- pH adjusted: no
- Suspended solids concentration: 30 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 3
- Measuring equipment:
Oxygen electrode - Range 0 to 10 mg/l with a precision of 1%. Steady-state is reached within 1.5-2 min.
- Method used to create aerobic conditions: air diffuser for 15min, using water-washed air.

SAMPLING
- Sampling frequency: weekly
- Sampling method: After each one-week's incubotion period, the concentration of dissolved oxygen in each bottle is determined. The pH is measured. The medium in each bottle is aerated with an air-diffuser for 15 min. at 20 + 0.5 °C. The bottles are re-stopped and incubated on the shaking device,

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (three replicates)
- Three bottles of the reference substance (aniline)
- Toxicity control: yes (one bottle, 15.5 mg/L test substance/reference substance stock solution)

Reference substance

Reference substance:

aniline

Results and discussion

Test performance:

The total oxygen uptake of the blank bottles after the first week of the test is lower than 3 mg O2 and during the following weeks < 1 mg O2 per week. The percentage degradation of the reference substance aniline is > 60% by day 14.

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Remarks:

V190110

Value:

-7

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Remarks:

V190110 and aniline

Value:

-3

Sampling time:

28 d

Remarks on result:

other:

Remarks:

This means that the test substance has an inhibitory effect at the tested concentration

% Degradation 3

Parameter:

% degradation (O2 consumption)

Remarks:

aniline

Value:

80

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

V190110 had an inhibitory effect on biodegradation at the tested concentration.