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EC number: 235-795-5 | CAS number: 12738-64-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- not relevant
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Remarks:
- See abov
- Vehicle:
- not specified
- Details on test solutions:
- Stock solutions (0.5 to 5.0 g/L) of the test chemicals were prepared in deionized water prior to inhibition testing. When necessary, the stock solutions were adjusted to pH 7.5±0.5 by the addition of 1N H2SO 4 or IN NaOH as required. 3,5-Dichlorophenol was included as a reference compound in each inhibition test. Stock solutions were prepared by dissolving 0.5 g of 3,5-dichlorophenol in 10 mL of IN NaOH and diluting to 30 mL with deionized water. The solution was adjusted to pH 7.5±0.5 by the addition of 8 mL of 1N H2SO 4, and then diluted to a total volume of 1 liter with deionized water.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge was obtained from a local municipal wastewater treatment plant on the day prior to the first day of each series of inhibition tests. For a few of the latter studies, activated sludge was also obtained from a local industrial wastewater treatment plant. On return to the laboratory, the solids were allowed to settle and the waste liquor was discarded. The solids were then transferred into a 9-1iter laboratory-scale, semicontinuous activated sludge cylinder and diluted to approximately 8.5 liters with deionized water. The system was initially mixed by aerating at a rate of approximately 0.5 L/min, and the concentration of mixed liquor suspended solids was determined by gravimetric analysis. The solids were then allowed to settle in the cylinder and the upper layer of waste liquor was discarded. The activated sludge was washed three times with appropriate volumes of deionized water. After washing, the system was adjusted to contain 4000±400 mg of mixed liquor suspended solids (dry weight) per liter. The system was then aerated continuously at a rate of 0.5 L/min and incubated at ambient temperature (21°C).
The system was supplemented daily with 50 mL of a synthetic sewage stock solution per liter of activated sludge. The synthetic sewage stock solution was composed of, per liter:
Bacto-Peptone, 16.0 g; Bacto-Beef extract, 11.0 g; urea, 3.0 g; K2HPO4 , 28.0 g; MgSO4.7H20, 0.2 g; CaCI2.2H20 , 0.4 g; and NaCl, 0.7 g. Final pH of the stock solution was adjusted to pH 7.0 with H3PO 4. The synthetic sewage stock solution was similar to that described by the OECD with the exception that the level of K2HPO4 was increased to 28.0 g for increased buffering capacity. Fresh stock solution was prepared as required and stored for not more than two days at 5°C. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- Post exposure observation period:
- no
- Hardness:
- No data
- Test temperature:
- 21 oC
- pH:
- 7.5 ± 0.5
- Nominal and measured concentrations:
- Nominal
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Confidence interval: 593-697
- Results with reference substance (positive control):
- The results obtained with the reference item 3,5-dichlorophenol, showed an EC50 12.4 mg/L (180 min). The EC50 was within the 2 to 25 mg/L range, and it was considered acceptable for the test
- Validity criteria fulfilled:
- yes
- Conclusions:
- 180 min EC50 > 1000 mg/L (respiration rate)
- Executive summary:
The test item was tested according to OECD Guideline no 209: Activated Sludge, Respiration Inhibition Test. The study was conducted according to GLP.
Resulting EC50 >1000 mg/L (180 min).
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Read-across toxicity studies on one closely structural and physicochemical related substance: Benzoic acid.
For further read-across justification se document attached in section 13 - Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on a read-across to benzoic acid, the EC50(activated sludge, 3hr) is derived at >1000 mg/L
- Executive summary:
Based on a read-across to benzoic acid, the EC50(activated sludge, 3hr) is derived at >1000 mg/L
Referenceopen allclose all
No data
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Based on a read-across to benzoic acid, the EC50(activated sludge, 3hr) is derived at >1000 mg/L
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