12-hydroxy-N-(2-hydroxyethyl)octadecan-1-amide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
2.This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio).
3.Bioassay was conducted to evaluate toxicity of test material on fish.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

2. The test substance is insoluble in water as well as in the solvents namely, ethanol, acetone, acetonitrile, N,N-Dimethyl formamide, Tertiary butyl alcohol, chloroform. Therefore, the test substance was used as it is and test solution was prepared by dissolving 50 mg, 100 mg, & 150 mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, respectively.

Test organisms (species):

other: 2. Danio rerio , 3.Oryzias latipes

Details on test organisms:

.TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Ashish Fish Center, Dharampeth, Nagpur, MS (India)
- Age at study initiation (mean and range, SD): 3-4 months
- Length at study initiation (length definition, mean, range and SD): Average 3.63 cm
- Weight at study initiation (mean and range, SD): 0.399 g (approx.)
- Method of breeding: No data
- Feeding during test: Food was withdrawn 1 day before and during exposure
- Food type: Standard brand feed
- Frequency: Once daily

ACCLIMATION
- Acclimation period: seven days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Standard brand feed
- Feeding frequency: Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed): During last 48 hours mortality was < 5 %

2.The fish were held in the laboratory at 20-23oC for 2-4 weeks prior to use in bioassay and were fed an excess of dry commercial diet pellet for trout daily. The Ssh were acclimated to the test system for 2 days prior to testing without feeding.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2)25 milligrams per liter Calcium ca

Test temperature:

21- 36°C

pH:

6-8

Dissolved oxygen:

1) 7.4 mg/l
2)≥5-≥8.5 milligrams per liter

Nominal and measured concentrations:

1) 5 mg/L, 10 mg/L & 15 mg/L

Details on test conditions:

1) TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12 inch each.
- Type: open
- Material, size, headspace, fill volume:
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water was used

OTHER TEST CONDITIONS
Photoperiod: 12 hours light and 12-hour darkness

2)Ten fish were tested at each concentration which l'ncreases by geometrical progression of 1.4 in 3-5 / glass beaker held in constant temperature (21-22oC) water bath without feeding or artihcial aeration.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

The 96 hr LC50 value was evaluated to be >15 mg/l.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:

For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.

For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L.

Thus, during the exposure period of 96 hr, test chemical was considered as non-toxic at environmental relevamt concentrations.

Description of key information

Short term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:

For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.

For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L.

Thus, during the exposure period of 96 hr, test chemical was considered as non-toxic at environmental relevamt concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

15 mg/L

Additional information

Short term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:

For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.

For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L.

Thus, during the exposure period of 96 hr, test chemical was considered as non-toxic at environmental relevamt concentrations.