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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: Test guideline under Japan Chemical Substances Control Law
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts
EC Number:
812-037-7
Cas Number:
1793011-72-9
Molecular formula:
Unknown for all components
IUPAC Name:
Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts
Test material form:
solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Mixed Liquor Suspended Solids (MLSS): 2450 mg/L (BOD measurement and recovery test)
Source: Chemical Evaluation and Research Institute, Japan
Date of Receipt: 2017-01-06
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
DOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
Temperature: 25 ± 1°C
Test volume: 300 mL

Test bottle contents :
Bottle 1 : Test substance + ultrapure water (Abiotic sterile control)
Bottle 2-4 : Test substance + activated sludge + basal medium (test suspension)
Bottles 5 : Activated sludge + aniline + basal medium (Positive control)
Bottle 6 : Basal medium (control)

The BOD was measured continuously for 28 days.
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
From the biodegradability result based on BOD (average 0%), that of the residual test item remaining (average 5%), and the DOC (average 12%) it is concluded that the test substance is not readily biodegradable. Therefore, no transformation products were generated under the conditions of the test.
% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Parameter:
% degradation (test mat. analysis)
Value:
5
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
12
Sampling time:
28 d
Details on results:
-Degradability based on BOD
The degree of degradability based on the BOD measurements was 0, 0, and 0% for bottles 2, 3, and 4, respectively.
-Degradability based on DOC
The degree of degradability based on the DOC measurements was 11, 13 and 13% for bottles 2, 3 and 4, respectively.
-Degradability based on the residual test substance concentration
The degree of degradability based on the residual test substance concentration was 10, 1, and 5 % for bottles 2, 3 and 4, respectively.

At the end of the experiment, the pH values were 9.0 for test substance suspension bottles with no sludge and 7.3 for test substance bottles with sludge.

BOD5 / COD results

Results with reference substance:
The degradability of aniline, based on the BOD measurements was 80 % after 7 days. The activity of the sludge was thus shown to be satisfactory.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
It is concluded that the test substance was not readily biodegradable and no transformation products were generated under the conditions of the modified MITI test.