N,N-bis(2-hydroxyethyl)undec-10-enamide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From March 03, 2011 to March 09, 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Fresh sample of activated sludge from the aeration basin of a municipal sewage treatment plant (Moers, Germany)
- Filtered sample of 1875 mg suspended solids
- Following washing and dilutions in a mineral medium, the amount of inoculum corresponded to 30 mg/L suspended solids.

Duration of test (contact time):

ca. 28 d

Initial conc.:

100 mg/L

Based on:

COD

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

percentage oxygen uptake of the COD

Details on study design:

The experiment was carried out in an closed incubation device, the Sensomat system at ca. 22°C. Biotic and abiotic (control) degradation was measured.

Reference substance:

benzoic acid, sodium salt

Remarks:

at 100 mg/L

Test performance:

Measurement of the percentage of COD/ThOD NH4 (ThOD NH4 = theoretical oxygen demand assuming no nitrification occurs)

Key result

Parameter:

other: % of COD/ThOD NH4

Value:

ca. 97

Sampling time:

28 d

Remarks on result:

other: readily biodegradable

Details on results:

- The average biodegradation value of the test substance was 97% after 28 days. 83 % biodegradation accured within a 10-d window during the 28-d test period.
- The average degradation value of the abiotic control was -1% after 28 days at a concentration of 100 mg test substance/L.
- The test substance is not assumed to be inhibitory, because 84% degradation degradation occured with 14 days in the toxicity control preparations containing 100 mg test substance and mg sodium benzoate per litre.
- Inoculum blanks reached a mean oxygen consumption of 19.7 mg/L with 28 days. Unusual observations were not made.

Results with reference substance:

Sodium benzoate was biodegradated to an average of 90% within 14 days.

None.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Under the study conditions, the test substance was considered to be readily biodegradable.

Executive summary:

A study was conducted to determine the ready biodegradability of the test substance, C11-unsatd. DEA (>75% active), according to OECD Guideline 301F and EU Method C.4 D (manometric respirometry test), in compliance with GLP. Activated sludge (amount of inoculum: 30 mg/L suspended solids) was exposed for 28 d to the test substance at a concentration of 100 mg/L (based on COD). The experiment was carried out in a closed incubation device, the Sensomat system at ca. 22°C. Biotic and abiotic (control) degradation was measured. More especially, the biodegradation was assessed by the measurement of the percentage of COD/ThOD NH4, assuming no nitrification occurs. A negative (blank) and a positive (sodium benzoate) control were included in the experiment. The average biodegradation value of the test substance was 97% after 28 d. 83% biodegradation occurred within a 10 d window during the test period. The average degradation value of the abiotic control was -1% after 28 d at a concentration of 100 mg test substance/L. The test substance was not assumed to be inhibitory, because 84% degradation occurred with 14 d in the toxicity control preparations containing 100 mg test substance and mg sodium benzoate per litre. Finally, inoculum blanks reached a mean oxygen consumption of 19.7 mg/L with 28 d and unusual observations were not made. Sodium benzoate was biodegraded to an average of 90% within 14 d. The experiment fulfilled the validity criteria. Under the study conditions, the test substance was considered to be readily biodegradable (Mey, 2011).

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

A study was conducted to determine the ready biodegradability of the test substance, C11-unsatd. DEA (>75% active), according to OECD Guideline 301F and EU Method C.4 D (manometric respirometry test), in compliance with GLP. Activated sludge (amount of inoculum: 30 mg/L suspended solids) was exposed for 28 d to the test substance at a concentration of 100 mg/L (based on COD). The experiment was carried out in a closed incubation device, the Sensomat system at ca. 22°C. Biotic and abiotic (control) degradation was measured. More especially, the biodegradation was assessed by the measurement of the percentage of COD/ThOD NH4, assuming no nitrification occurs. A negative (blank) and a positive (sodium benzoate) control were included in the experiment. The average biodegradation value of the test substance was 97% after 28 d. 83% biodegradation occurred within a 10 d window during the test period. The average degradation value of the abiotic control was -1% after 28 d at a concentration of 100 mg test substance/L. The test substance was not assumed to be inhibitory, because 84% degradation occurred with 14 d in the toxicity control preparations containing 100 mg test substance and mg sodium benzoate per litre. Finally, inoculum blanks reached a mean oxygen consumption of 19.7 mg/L with 28 d and unusual observations were not made. Sodium benzoate was biodegraded to an average of 90% within 14 d. The experiment fulfilled the validity criteria. Under the study conditions, the test substance was considered to be readily biodegradable (Mey, 2011).