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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21.06.2004-07.07.2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 835.1110 (Activated Sludge Sorption Isotherm)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
other: reversed phase column packed with a commercial\y available cyanopropyl solid phase containing lipophilic and polar moieties
Radiolabelling:
no
Details on study design: HPLC method:
Reagents
Acetonitrile, Chromasolv Riedel de Haen
Methanol, Chromasolv Riedel de Haen
Cleaned water (laboratory equipment)
Distilled water, Roth
Dead time marker: Formamide, Merck
Calibration substances (Merck, Riedel de Haen, Table 2 and 4)

Instruments
Chromatographie apparatus refer chapter 4.4
Analytical balance Sartorius Rrn·o P
Ultrasonic bath Branson 3200
Variable pipettes, Brand
General laboratory glassware

HPLC equipment: HPLC pump G1312A, autosampler G1313A, column oven G1316A, degaser G1322A and Diode Array Detector G1315B by Agilent
Column: stationary phase: LiChrospher 100 CN 5μm by Duratec (9911-02)
material: stainless steel, dimensions: 250 mm x 4.6 mm
column temperature: 25 °C
Mobile Phase: methanol / cleaned water 75:25 % (v/v), isocratic elution
Flow rate: 1.0 ml/min
lnjection volume: 10 μI
Detection wavelength: 210 nm (dead time marker and calibration substances)
230 nm (lest item)
Injection amounts:
Dead time marker: 17.4 μg
Calibration substances: 0.68-1.93 μg
Testitem: 0.49 μg

The dead time marker and the calibration substances were dissolved in acetonitrile and diluted 0.1 to 1 in eluent and injected as single standards twice. The item was dissolved in methanol and diluted 0.1 to 1 in eluent and injected as single solution twice.
Key result
Sample No.:
#1
Type:
log Koc
Value:
ca. 3.448 dimensionless
pH:
7
Temp.:
25 °C
Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient of the test item was found to be: log Koc 3.4
Executive summary:

The adsorption coefficient of the test item was determined according to the "HPLC-method" described in guideline 67/548/EEC, annex C.19.

The adsorption coefficient of the test item was found to be: log Koc 3.4

Description of key information

The adsorption coefficient of the test item was found to be: log Koc 3.4

Key value for chemical safety assessment

Koc at 20 °C:
2 511.89

Additional information