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Registration Dossier
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EC number: 454-790-0 | CAS number: -
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21.06.2004-07.07.2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.1110 (Activated Sludge Sorption Isotherm)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- other: reversed phase column packed with a commercial\y available cyanopropyl solid phase containing lipophilic and polar moieties
- Radiolabelling:
- no
- Details on study design: HPLC method:
- Reagents
Acetonitrile, Chromasolv Riedel de Haen
Methanol, Chromasolv Riedel de Haen
Cleaned water (laboratory equipment)
Distilled water, Roth
Dead time marker: Formamide, Merck
Calibration substances (Merck, Riedel de Haen, Table 2 and 4)
Instruments
Chromatographie apparatus refer chapter 4.4
Analytical balance Sartorius Rrn·o P
Ultrasonic bath Branson 3200
Variable pipettes, Brand
General laboratory glassware
HPLC equipment: HPLC pump G1312A, autosampler G1313A, column oven G1316A, degaser G1322A and Diode Array Detector G1315B by Agilent
Column: stationary phase: LiChrospher 100 CN 5μm by Duratec (9911-02)
material: stainless steel, dimensions: 250 mm x 4.6 mm
column temperature: 25 °C
Mobile Phase: methanol / cleaned water 75:25 % (v/v), isocratic elution
Flow rate: 1.0 ml/min
lnjection volume: 10 μI
Detection wavelength: 210 nm (dead time marker and calibration substances)
230 nm (lest item)
Injection amounts:
Dead time marker: 17.4 μg
Calibration substances: 0.68-1.93 μg
Testitem: 0.49 μg
The dead time marker and the calibration substances were dissolved in acetonitrile and diluted 0.1 to 1 in eluent and injected as single standards twice. The item was dissolved in methanol and diluted 0.1 to 1 in eluent and injected as single solution twice. - Key result
- Sample No.:
- #1
- Type:
- log Koc
- Value:
- ca. 3.448 dimensionless
- pH:
- 7
- Temp.:
- 25 °C
- Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient of the test item was found to be: log Koc 3.4
- Executive summary:
The adsorption coefficient of the test item was determined according to the "HPLC-method" described in guideline 67/548/EEC, annex C.19.
The adsorption coefficient of the test item was found to be: log Koc 3.4
Reference
Description of key information
The adsorption coefficient of the test item was found to be: log Koc 3.4
Key value for chemical safety assessment
- Koc at 20 °C:
- 2 511.89
Additional information
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