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EC number: 454-790-0 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 30 - Nov 27, 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- EEC Publication No. L 383 A, December 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Origin:
activated sludge, micro organisms from a domestic waste water treatment plant supplied by the sewage treatment plant Groß-Zimmern, Germany
-Conditioning:
The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final. sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- aniline
- Test performance:
- Based on ThODNH4 the reference item aniline was sufficiently degraded to 81 % after 14 days, and to 96 % after 28 days of incubation.
The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance): 1 % degradation after 28 d
- Results with reference substance:
- Points of degradation plot (reference substance):
31 % degradation after 7 d
81 % degradation after 14 d
91 % degradation after 21 d
96 % degradation after 28 d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
- Executive summary:
Purpose
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.The GLP study was performed according to OECD TG 301F.
Result
Biodegradation of test item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period degradation rates of -1% and 1% based on ThOD(NH4) were found.
Biodegradation of Aniline
The reference item Aniline was sufficiently degraded to 81 % after 14 days, and to 96 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Biodegradation in the Toxicity Control
In the toxicity control containing both, the test item and the reference item aniline, 44 % biodegradation was noted within 14 days and 56 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.
Conclusion
The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Reference
Description of key information
The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
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