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EC number: 932-164-2 | CAS number: -
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 20 September 2007 to 27 September 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- An amount of reference material (160 mg) was dissoled in water with the aid of ultrasonication for approximately 20 minutes and the volume adjusted to 1litre to give a 160 mg/L stock solution. Aliquots (10 and 100 ml) of the 160 mg/L stock solution were removed and dispersed with activated sewage sludge and water to give the final concentrations of 3.2 and 32 mg/L. the volumetric flask containing the reference material was inverted several time to ensure homogeneity of the solution.
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Loughborough, Leicestershire, UK
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- 140 mg/L as CaCO3
- Test temperature:
- 21ºC
- pH:
- Maximum 8.7
- Dissolved oxygen:
- initial O2 Reading: 8.7 mg O2/L
O2 Consumption Rates: 0.53 mg O2/L/min - Details on test conditions:
- TEST SYSTEM
An amount of test material (2000 mg) was dispersed in water with the aid of ultrasonication for approximately 15 minutes and the volume adjusted to 1 litre to give a 2000 mg/L stock dispersion. An aliquot (250 mL) of this stock dispersion was dispersed with synthetic sewage (16 ml), activated sewage sludge (200ml) and water, to a final volume of 500 ml, to give the required concentration 1000 mg/l. The volume flask contained the stock dispersion was inverted several times to ensure homogeneity of the stock dispersion.
This method of preparation was performed for the three separate replicate test vessels.
Synthetic Sewage:
Peptone: 16 g
Meat extract: 11 g
Urea: 3 g
NaCl: 0.7 g
CaCl2.2H20: 0.4 g
MgSO4.7H2O: 0.2 g
K2HPO4: 2.8 g
dissolved in 1L of water with the aid of ultrasonication
- Reference substance (positive control):
- yes
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 30 min
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Remarks on result:
- other: 7.0-11 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Results with reference substance (positive control):
- EC 50 (3 hours)= 8.9 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the test material on the respiration sewage sludge micro-organisms gave a 3-Hour EC50 of greater than 1000 mg/L. The NOEC after 3 hours exposure was 1000 mg/L
- Executive summary:
A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD 209.
Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test material at a concentration of 1000 mg/L (three replicates flasks) for a period of 3 hours at a temperature of approximately 21ºC with the addition of a synthetic sewage as a respiratory substance.
The rate of respiration was determined after 30 minutes and 3 hours contact time and compared to data for the control and a reference material, 3,5 -dichlorophenol.
The effect of the test material on the respiration of activated sewage sludge gave a 3 -Hours EC50 of greater than 1000 mg/L. NOEC after 3 hours exposure was 1000 mg/L.
It was considered unnecessary and unrealistic to test at concentration in excess of 1000 mg/L.
The reference material gave a 3 -Hour EC50 value of 8.9 mg/L, 95% confidence limits 7.0 -11 mg/L.
Reference
ECx (30 minutes) (mg/L) |
95% Confidence Limits (mg/L) | ECx (30 minutes) (mg/L) |
95% Confidence Limits (mg/L) | |
EC20 | >1000 | - | >1000 | - |
EC50 | >1000 | - | >1000 | - |
EC80 | >1000 | - | >1000 | - |
NOEC | 1000 | - | 1000 | - |
Description of key information
A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD 209.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The effect of the test material on the respiration of activated sewage sludge gave a 3 -Hours EC50 of greater than 1000 mg/L. NOEC after 3 hours exposure was 1000 mg/L.
It was considered unnecessary and unrealistic to test at concentration in excess of 1000 mg/L.
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