Oligomerisation products of ethylene oxide with reaction products of rape oil and ethanolamine

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15/10/2007-08/01/2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

not specified

Buffers:

Specifications of buffer solutions (see table)
The buffer solutions were filtered through a 0.2 µm membrane filter to ensure they were sterile before commencement of the test. Also these solutions were subjected to ultrasonication and degassing with nitrogen to minimise dissolved oxygen content.

Duration:

433 d

pH:

4

Temp.:

25 °C

Preliminary study:

pH Rate constant (s^-1) Estimated half-life at a 25ºC
4 1.85 x 10^-8 433 days
7 1.03 x 10^-7 77.6 days
Conclusion: the preliminary test shows that the substance is hydrolytically sufficient stable to not do the main test.
9 Not applicable >1 year

Transformation products:

not specified

Key result

pH:

4

Temp.:

25 °C

Hydrolysis rate constant:

0 s-1

DT50:

433 d

Key result

pH:

7

Temp.:

25 °C

Hydrolysis rate constant:

0 s-1

DT50:

77.6 d

Key result

pH:

9

Temp.:

25 °C

DT50:

> 1 yr

Remarks on result:

not determinable because of methodological limitations

pH	Rate constant (s^-1)	Estimated half-life at a 25ºC
4	1.85 x 10^-8	433 days
7	1.03 x 10^-7	77.6 days
9	Not applicable	>1 year

Validity criteria fulfilled:

yes

Remarks:

The linearity of the detector response with respect to concentration was assessed over the nominal concentration range of at least 0 to 1.00 mg/L for each of the analytical matrixes used

Conclusions:

The rate constant and estimated half-life at 25ºC of the test material are shown in the following table (see executive summary)

Executive summary:

Assessment of hydrolytic stability was carried out using Method 111 of the OECD Guidelines for Testing of Chemical, 13 April 2004. The results are as follows:

pH	Rate constant (s^-1)	Estimated half-life at a 25ºC
4	1.85 x 10^-8	433 days
7	1.03 x 10^-7	77.6 days
9	Not applicable	>1 year

Additional testing at pH 1.2, 37.0 ± 0.5, resulted in a second order correlation for the reduction in the test material concentration with time. On analysis after incubation periods of 1, 3, 5 and 24 hours, 88.9, 82.3, 81.0 and 65.9% of the initial concentration remained respectively.

Description of key information

Assessment of hydrolytic stability was carried out using Method 111 of the OECD Guidelines for Testing of Chemical, 13 April 2004. 

Key value for chemical safety assessment

Half-life for hydrolysis:

77.6 d

at the temperature of:

25 °C

Additional information

Assessment of hydrolytic stability was carried out using Method 111 of the OECD Guidelines for Testing of Chemical, 13 April 2004. Value is for hydrolysis at pH 7.