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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-05-08 to 1991-05-22
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil: The test item was applied as dry sand premixes. The dose concentrations were calculated to give the same weight of sand at all dose levels. For each group the premix was thoroughly mixed with a small proportion of soil prior to incorporation with the main soil bulk. Water was gradually mixed with the treated soil to give a moisture content equivalent to 35 % of the dry weight.
- Controls: Negative control soil was treated with an equivalent weight of dry sand.


Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: Local Supplier, Houghton, St. Ives, Cambridgeshire, England, UK
- Age at test initiation: adult with clitella
- Weight at test initiation: 529 - 643 mg

ACCLIMATION
- Acclimation period: 1 day
- Acclimation conditions: same as test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Test temperature:
20 - 21 °C
pH:
5.7 (addition of calcium carbonate)
Moisture:
35 % of the dry weight
Details on test conditions:
TEST SYSTEM
- Test container (material, size): glass container (1 L)
- Amount of soil or substrate: 729 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE
- Composition (% w/w):
Quartz sand 70
Kaolin clay 20
Sphagnum peat 10

OTHER TEST CONDITIONS
- Photoperiod: continous
- Light intensity: 400 lux

EFFECT PARAMETERS MEASURED:
Counts of worms, on day 7 and 14
Health and mortalities: Daily
Weights: on day 0 and 14

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Test concentrations: 0, 95, 171, 309, 556, 1000 ppm
Nominal and measured concentrations:
0, 95, 171, 309, 556, or 1000 ppm
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period:
No mortalities were detected at the day 7 count. By day 14, one mortality had occurred in the 309 ppm dose group. This mortality was not considered to be treatment-related. No mortalities occurred in any other group.
- Changes in body weight of live adults at end of exposure period: Weight losses were observed in all groups. However, there was no clear evidence of any treatment-related effect.
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: The LC50 values for chloroacetamide, tested previously as a positive control between 15 and 29 May 1985 were found to be:
Day 7 LC50: 43.1 ppm (95 % confidence limits 34.0 – 57.6 ppm)
Day 14 LC50: 24.6 ppm (95 % confidence limits 20.2 – 30.5 ppm)
Reported statistics and error estimates:
As outlined in OECD Method No. 207

Table 1: Weight Development

Treatment

Concentration (ppm)

Weight (mg)

% Decrease

Day 0

Group mean

Day 14

Group mean

Neg. Control

0

643

592

478

451

23.8

529

428

596

442

600

456

Test Item

95

639

602

478

489

23.8

601

460

572

439

595

459

Test Item

171

605

602

470

479

20.4

582

432

628

488

594

525

Test Item

309

582

603

447

488

19.1

594

528

623

496

613

485

Test Item

556

584

603

492

498

17.4

594

497

614

508

620

496

Test Item

1000

615

601

486

474

21.1

615

490

587

449

585

471

Validity criteria fulfilled:
yes
Conclusions:
It was not possible to determine the LC50 values of ODB-2 to the earthworm at Days 7 and 14. The LC50 is above 1000 ppm, the maximum treatment level tested.
Executive summary:

In a 14 -day acute toxicity study, earthworms (Eisenia fetida) were expsoed to the test substance at 0, 95, 171, 309, 556, or 1000 ppm (Huntingdon, 1991). The reference chemical used was chloroacetamide. The experiment was carried out in accordance with OECD guideline 207.

No mortalities were detected at the day 7 count. By Day 14, one mortality had occurred in the 309 ppm dose group. This mortality was not considered to be treatment-related. No mortalities occurred in any other group. Weight losses were observed in all groups. However, there was no clear evidence of any treatment-related effect.

The LC50 values for chloroacetamide, tested previously as a positive control were found to be:

Day 7 LC50: 43.1 ppm (95 % confidence limits 34.0 – 57.6 ppm)

Day 14 LC50: 24.6 ppm (95 % confidence limits 20.2 – 30.5 ppm)

Because of the limited number of mortalities it was not possible to determine the LC50 values of the test item to the earthworm at Days 7 and 14. These values must lie in excess of 1000 ppm, the maximum treatment level used. The test substance is therefore regarded as non-toxic to earthworms above a concentration of 1000 ppm.

Description of key information

It was not possible to determine the LC50 values of the test substance to the earthworm on days 7 and 14. The LC50 is above 1000 ppm, the maximum treatment level tested.

Key value for chemical safety assessment

Additional information

In a 14 -day acute toxicity study, earthworms (Eisenia fetida) were exposed to the test substance at 0, 95, 171, 309, 556, or 1000 ppm (Huntingdon, 1991). The reference chemical used was chloroacetamide. The experiment was carried out in accordance with OECD guideline 207.

No mortalities were detected at the day 7 count. By day 14, one mortality had occurred in the 309 ppm dose group. This mortality was not considered to be treatment-related. No mortalities occurred in any other group. Weight losses were observed in all groups. However, there was no clear evidence of any treatment-related effect.

The LC50 values for chloroacetamide, tested previously as a positive control were found to be:

Day 7 LC50: 43.1 ppm (95 % confidence limits 34.0 – 57.6 ppm)

Day 14 LC50: 24.6 ppm (95 % confidence limits 20.2 – 30.5 ppm)

Because of the limited number of mortalities it was not possible to determine the LC50 values of the test substance to the earthworm on days 7 and 14. These values must be above 1000 ppm, the maximum treatment level used.

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