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EC number: 403-830-5 | CAS number: 89331-94-2 B 290; BK 400; CK 34; DIBUTYL-N-102; DX-20; FAT NR. 40391/A; FLUORAN BLACK BD 869; FLUORAN SCHWARZ BD 869; NOIR FLUORANE BD 869; ODB-2; PSD-290; SENOR-2; TG-31; TH-108; WINCON-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-10-31 to 1988-11-04
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: chemical analysis at 0, 24 and 96 hours.
- Vehicle:
- yes
- Remarks:
- 1 % Tween-80
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preliminary Solution in 1 % Tween 80-tetrahydrofuran. 100 mg test substance was dissolved in auxiliary solvent and the volume made up to 10 mL. 4 mL of this stock solution was added to 40 litre diluent to give the required test concentration.
1.0 mg/L was the highest test concentration due to the limited solubility of the test item and the restricted use of auxiliary solvents according to OECD Guideline 203. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Westacre Trout Farm, Norfolk, U.K.
- Length at study initiation ( mean and SD): 4.5 cm, SD = 0.2 cm
- Weight at study initiation (mean and SD): 1.01 g; SD = 0.16 g
ACCLIMATION
- Acclimation period: 8 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Commercial trout pellets
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): < 1 % in 7 days prior to the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 350 mg/L (as CaCO3)
- Test temperature:
- 14 ± 1 °C
- pH:
- 8.4
- Dissolved oxygen:
- 9.8 mgO2/L
- Nominal and measured concentrations:
- nominal: 1 mg/L
measured: 0.96 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Aquaria
- Type: open
- Material, fill volume: Glas, 40 L
- Aeration: Yes, via narrow bore glass tubes.
- Renewal rate of test solution: Daily
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.25 g bw/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water, dechlorinated by the addition of sodium thiosulphate.
- Total organic carbon: 4.5 mg/L; SD 0.51
- Metals:
Calcium: 133.5 mg/L; SD 0.95
Magnesium: 8.3 mg/L; SD 0.45
Sodium: 43.8 mg/L; SD 4.06
Potassium: 7.75 mg/L; SD 0.29
Iron: 0.0376 mg/L; SD 0.02
- Chlorine: 59.46 mg/L; SD 4.35
- Alkalinity: 215.2; SD 8.95
- Ca/Mg ratio: 16.6
- Period of water quality measurement: 1988-01-01 to 1988-03-31
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: light /dark 16 h/8 h
EFFECT PARAMETERS MEASURED: Mortality
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- not determinable
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- A 96-hour LC50 value of above 1.0 mg/L was determined. The NOEC was set to equal or above 1.0 mg/L.
- Executive summary:
Acute aquatic toxicity in fish was determined according to OECD guideline 203 (Huntingdon, 1989). Rainbow trout were exposed to the test substance under semi-static conditions for 96 hours. Due to the limited solubility of the test substance in water, 1 mg/L was the highest nominally tested concentration that could be prepared. No mortalities or sublethal effects were observed during the study. A 96-hour LC50 value of above 1.0 mg/L was determined. The NOEC was set to equal or above 1.0 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Test organisms (species):
- Oryzias latipes
- Test type:
- static
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In an acute fish test an LC50 >100 mg/L was observed after 96 h.
- Executive summary:
Short term aquatic toxicity was determined according to EU method C.1 (NIPPON SODA, ECHA RSS, 2017). Oryzia latipes was exposed to the test substance for 96 hours under static conditions. No effects were observed up to 100 mg/L (nominal). An LC50 of above 100 mg/L was determined.
Referenceopen allclose all
Description of key information
In the acute toxicity key study with fish (Oncorhynchus mykiss) an LC50 of above1 mg/L was determined for the test substance.
Key value for chemical safety assessment
Additional information
Key study
Acute aquatic toxicity in fish was determined according to OECD guideline 203 (Huntingdon, 1989). Rainbow trout were exposed to the test substance under semi-static conditions for 96 hours. Due to the limited solubility of the test substance in water, 1 mg/L was the highest nominally tested concentration that could be prepared. No mortalities or sublethal effects were observed during the study. A 96-hour LC50 value of above 1.0 mg/L was determined. The NOEC was set to equal or above 1.0 mg/L.
In accordance with Article 25(3) of the REACH Regulation the robust study summaries submitted 12 years previously, are additionally used for the purpose of the registration.
Supporting study
Short term aquatic toxicity was determined according to EU method C.1 (NIPPON SODA, ECHA RSS, 2017). Oryzia latipes was exposed to the test substance for 96 hours under static conditions. No effects were observed up to 100 mg/L (nominal). An LC50 of above 100 mg/L was determined.
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