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EC number: 947-119-2
CAS number: -
A guinea pig sensitization test following
the Buehler procedure (EPA Guideline 81-6) was conducted with NASUL 729.After
establishing the highest non-irritating dose concentration to be100%,a
induction period was initiated during which10young
adult male guinea pigs were treated with the test substanceand
10were treated with 0.08%Dinitrochlorobenzene
(DNCB) in 80%ethyl
alcohol (positive controls). During the induction period the animals
were dosed once week until a total of three dose applications was
achieved. Fourteen days after the third application a challenge dose was
applied to anaive site on each
24, 48 and72hours
later the animals were scored for a sensitization
response (erythema and edema). A naive control group of five animals was
maintained under the same environmental conditions and treated with the
test substance at challenge only. Because of the positive response
observed in the naive animals after challenge, the animals from all
groups were allowed to rest for 7 days
and were then re-challenged. A second group of naive animals was also
dosed at this time.
pigs appeared active and healthy throughout the test period. There were
no signs of gross toxicity, adverse pharmacologic effects or abnormal
behavior. All animals gained weight.
faint erythema and edema were observed at 1 test site 24 hours after the
first inductions. Virtually all animals exhibited erythema which varied
from very faint to moderate after the second and third inductions. Edema
was also noted at almost all sites after the second and third induction.
exhibited erythema 24 hours after the first induction and all had faint
to moderate-severe erythema with faint edema after the
test sites exhibited a positive response 24 and 48 hours after
challenge. At one site moderate erythema with edema was noted. Most
other sites exhibited only faint erythema. By 72 hours all signs of
site exhibited very faint erythema at 24
hours postchallenge. By 72 hours
erythema had cleared from the site. No irritation was noted atanyother
had a faint to moderate erythematous response24and48hours
post-challenge. Nine sites were calculated to be positive. Most
exhibited edema. By72hours
the incidence of positive reactions decreased to4.
Thelevel of severityremainedfaint
test sites hadapositive
response at 24 hours
after re-challenge. The severity of erythema was very faint
non-confluent to faint confluent. By 72 hours very faint erythema
remained at most of these sites.
irritation was noted atanyof
the naive control sites after rechallenge.
positive responses were observed at 24
and 48 hours after the second
challenge. Erythema varied in severity from very faint to
72 hours eight sites were positive.
these findings, NA-SUL 729,#3158 is considered to be a minimal contact
as received, undiluted, once a weekfor3
weeks. The positive response to 0.08% DCNB in 80% Ethyl Alcohol
validates the test system used in this study.
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