1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, no deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

EC Number:

611-799-6

Cas Number:

59121-99-2

Molecular formula:

C15H24O

IUPAC Name:

1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

Test material form:

liquid: viscous

Details on test material:

- Name of test material (as cited in study report): Vinylpseudoionol
- Molecular formula (if other than submission substance): C15H24O
- Molecular weight (if other than submission substance): 220.36 g/mol
- Physical state: viscous liquid
- Analytical purity: 87.1 %
- Impurities (identity and concentrations): 1.2 % water
- Purity test date: not indicated
- Lot/batch No.: Technikumscharge M401
- Expiration date of the lot/batch: not indicared
- Storage condition of test material: ca. 4 °C under nitrogen
- Water solubility: poorly water soluble

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Inoculum:
Activated sludge from laboratory wastewater plants treating municipal sewage Concentration of dry substanec 1 g/L

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

6.3 - 6.5 before correction; 7.2 - 7.4 after correction

Dissolved oxygen:

> 2.5 mg/l during aeration; > 6.5 mg/L before measurement

Nominal and measured concentrations:

nominal: 0, 100 mg/L

Details on test conditions:

Preparation of the stock solution of the test substance: - none , direct addition
Preparation of the inoculum:
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated for 24 hours. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.

TEST SYSTEM
- Test vessel: Erleenmeyer vessel (nominal 250 ml)
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of organisms per vessel: 5 g/L dry substance
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: not relevant
- Light intensity: not relevant

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 30, oxygen consumption

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20: ca. 3.3 mg/L
EC50: ca. 20 mg/L