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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11.5.95 - 8.6.95
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non-GLP
Remarks:
non-GLP
Justification for type of information:
The study is suitable to fullfill data requests.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
Version / remarks:
1981
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,6-di-tert-butyl-4-nonylphenol
EC Number:
224-320-7
EC Name:
2,6-di-tert-butyl-4-nonylphenol
Cas Number:
4306-88-1
Molecular formula:
C23H40O
IUPAC Name:
2,6-di-tert-butyl-4-nonylphenol
Details on test material:
Isonox 232 (Phenol, 2,6-bis (1,1-dimethylethyl)-4-nonyl)
Clear colorless liquid
Storage: In original container at room temperature
Batch number not provided in report

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
100 mg dry mass/L non-adapted microorganisms from 10 different sewage treatment plants throughout England. The mixed sludge was fed daily with a 0.1% synthetic sewage and maintained on constant aeration at 25±2°C until use.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
30 mg/L
Based on:
test mat.
Initial conc.:
3.13 mg/L
Based on:
ThOD
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
The test was conducted in the dark. The results of the reference substance show the suitability of inoculum and culture conditions.
% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
31
Sampling time:
28 d

Applicant's summary and conclusion