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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1988

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Method: Draize Test
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Reference substance 002
Cas Number:
90817-34-8

Test animals / tissue source

Species:
rabbit
Strain:
other: New Zealand, Albino
Details on test animals or tissues and environmental conditions:
6 males
Acclimatization: at least 1 week unter test conditions without treatment
Body weight: approx. 4 kg
Number of animals/cage: 1
Diet: ad libitum (Altromin-Haltungsdiät 2023)
Water: tap water ad libitum
Room temperature: approx. 20-21°C
Relative humidity: approx. 45-55%
Light/dark cycle: 12/12 hours

Test system

Vehicle:
other: the test substance was suspended in carboxymethylcellulose (2%) and cremophor (0.5%)
Amount / concentration applied:
Concentration: 5 %
Amount applied: 0.1 ml
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
72 hours
Details on study design:
the eye was not rinsed, permanent contact

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 1, 6, 24, 48, 72 hours
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 1, 6, 24, 48, 72 hours
Score:
0
Max. score:
2
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 1, 6, 24, 48, 72 hours
Score:
0
Max. score:
3
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 1, 6, 24, 48, 72 hours
Score:
0
Max. score:
4

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information
Conclusions:
The 5% dilution of 3-Amino-2-methylamino-6-methoxy-pyridin has to be classified as non irritant or corrosive on the eyes.
Executive summary:

3-Amino-2-methylamino-6-methoxy-pyridin (Ro 730) was tested for primary eye irritation on rabbits by single application of 100 µl of a 5 % preparation of the substance in 2 % carboxymethylcellu­lose and 0.5 % cremophor and permanent contact. The pH-value was adjusted with undiluted ammonium at approx. 9. On the cornea, iris and conjunctivae were no reactions observed.

The 5% dilution of 3-Amino-2-methylamino-6-methoxy-pyridin has to be classified as non irritant or corrosive on the eyes.