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Toxicity to aquatic algae and cyanobacteria

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The algae are usually preferred as reference aquatic plants in a study of growth inhibition. However, coloured substances cause problems in interpreting test algae, because the effects of the absorption of light can interact with potential toxicity; therefore, the inhibition of the growth of algae would no longer be only the result of a toxic action, but also the result of physical phenomena [shading effect] (OECD guideline 221 according to the Guidance on Information Requirements and Chemical Safety Assessment Chapter R.7b: Endpoint specific guidance (Version 2.0 November 2014), published by the European Chemical Agency)