(R)-3,7-dimethyloct-6-enal

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EU Directive 79/831/EEC, Annex V, part C

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Citronellal

EC Number:

203-376-6

EC Name:

Citronellal

Cas Number:

106-23-0

Molecular formula:

C10H18O

IUPAC Name:

3,7-dimethyloct-6-enal

Details on test material:

- Name of test material (as cited in study report): 3.7-Dimethyl-6-octen-1-al
(Citronellal)
- Analytical purity: 97.6 %
Water solubility: > 500 mg/l at 20 °C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

DPREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of the test item of the highest test concentration (nominal 100 mg/l) was prepared using 100 mg/l Tween 80 as a solvent and intense stirring for 60 min at room temperature (20 °C). Then, adequate volumes of this stock solution were diluted with culture medium to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test
- Food type:
- Amount:
- Frequency:


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): Synthetic fresh water was used for culture and test purposes. The properties of this test medium were as follows: Total hardness: 2.70 +/- 0.50 mmol/l Alkalinity up to pH 4.3: 0.90 +/- 0.10 mmol/l Molar ratio Ca:Mg: about 4 : 1 pH value: 8.0 +/- 0.5 Conductivity: 600 - 700 µS/cm. After preparation the medium was aerated for approximately 24 h until saturation.
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19 - 21 °C, constant within +/- 1 °C

pH:

9.2 - 6.3

Dissolved oxygen:

8.7 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
3.905, 7.81, 15.6, 31.2, 62.5, 125, 250, 500, control, solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes with flat bottom (glass)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):4
- Biomass loading rate: 0.5 animals/ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11



OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime,
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Swimming ability of the test animals after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

3.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

8.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

% of immobile daphnids after x hours:
Nominal test concentration (mg/l) 
        /  0  /   3 /   6 /  24 /  48 /
Control /  0  /   0 /   0 /   0 /   0 /
.195    /  0  /   0 /   0 /   0 /   0 /
.39     /  0  /   0 /   0 /   0 /   0 /
.78     /  0  /   0 /   0 /   0 /   0 /
1.56    /  0  /   0 /   0 /   0 /   0 /
3.12    /  0  /   0 /   0 /   5 /   5 /
6.24    /  0  /   0 /   0 /  30 /  50 /
12.5    /  0  /   0 /  10 /  50 /  50 /
25.0    /  0  /  80 / 100 / 100 / 100 /
49.9    /  0  / 100 / 100 / 100 / 100 /
100.0   /  0  / 100 / 100 / 100 / 100 /
 
EC50 (48 h): 8.68 mg/l (CL 95 %: 6.97 - 10.39 mg/l).
EC0 (48 h):3.12 mg/l
EC100 (48 h):25 mg/l.

EC50 (24 h): 10.06 mg/l (CL 95 %: 8.17 - 11.95 mg/l).
EC0 (24 h): 3.12 mg/l
EC100 (24 h): 25 mg/l.

All results refer to nominal test concentrations.