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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 (48 h, Daphnia magna) > 100 mg/L (nominal)

Key value for chemical safety assessment

Additional information

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna, according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. For the test nominal concentration used (100 mg/L) and control group, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

No toxicity was observed and none of the animals was immobilised in the blank control. A positive control was tested in a current reference study to assure that the test conditions were reliable. At the beginning and at the end of the test, the content of the test item in the test solutions was determined. The measured concentration was 101 % and 112 % of the nominal at the start and at the end respectively. Therefore, the determination of the NOEC / LOEC / ECx was based on the nominal concentrations.