Registration Dossier

Administrative data

Description of key information

Skin Sensitization:

No sensitization reactions were observed in any of the volunteers. Hence, 4-methoxyphenylacetone can be considered to be not sensitizing to human skin.

Key value for chemical safety assessment

Skin sensitisation

Link to relevant study records
Reference
Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journals
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate skin sensitization potential of 4-methoxyphenylacetone on 25 human volunteers.
GLP compliance:
not specified
Type of study:
other: Human maximization test
Justification for non-LLNA method:
Not specified
Specific details on test material used for the study:
IUPAC name: p- methoxyphenylacetone
commen name:1-(p-Methoxyphenyl)-2-propanone
Molecular formula:C10H12O2
Molecular weight :164.203 g/mol
Substance type:organic
InChI:1S/C10H12O2/c1-8(11)7-9-3-5-10(12-2)6-4-9/h3-6H,7H2,1-2H3
Smiles: c1(ccc(OC)cc1)CC(C)=O
physical property: liquid
Species:
other: Human
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
no data available
Route:
epicutaneous, occlusive
Vehicle:
petrolatum
Concentration / amount:
4% in petroletum
Day(s)/duration:
48 hours
Adequacy of induction:
not specified
No.:
#1
Route:
epicutaneous, occlusive
Vehicle:
petrolatum
Concentration / amount:
4% in petrolatum
Day(s)/duration:
48 hours
Adequacy of challenge:
not specified
No. of animals per dose:
25 human volunteers
Details on study design:
No data available
Challenge controls:
No data available
Positive control substance(s):
not specified
Reading:
1st reading
Group:
test group
Dose level:
4% in petrolatum
Total no. in group:
25
Clinical observations:
No skin reaction were observed
Remarks on result:
no indication of skin sensitisation
Cellular proliferation data / Observations:
No skin reaction observed
Interpretation of results:
other: not skin sensitizing
Conclusions:
No sensitization reactions were observed in any of the volunteers. Hence, 4-methoxyphenylacetone can be considered to be not sensitizing to human skin.
Executive summary:

A maximization test was carried out to assess the dermal sensitization potential of 4-methoxyphenylacetone in humans.

4-methoxyphenylacetone was applied in concentration 4% in petrolatum to the skin of 25 human volunteers and observed till 48 hours.

No sensitization reactions were observed in any of the volunteers. Hence, 4-methoxyphenylacetone can be considered to be not sensitizing to human skin.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

Skin Sensitization:

Various studies have been investigated for assessing the dermal sensitization potential of 4-methoxyphenylacetone to a greater or lesser extent. The studies are based on in vivo experiments in guinea pigs along with predicted data for target chemical and its structurally similar read across; substances, 4-(p-methoxyphenyl) butan-2-one[CAS: 104-20-1] and 4-methoxybenzyl alcohol[CAS: 105 -13-5].The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

A maximization test was carried out (Food and Cosmetics Toxicology, Volume 17, Supplement, December 1979, Page 857) to assess the dermal sensitization potential of 4 -methoxyphenylacetone in humans. p-methoxyphenylacetone was applied in concentration 4% in petrolatum to the skin of 25 human volunteers and observed till 48 hours.

No sensitization reactions were observed in any of the volunteers. Hence, 4-methoxyphenylacetone can be considered to be not sensitizing to human skin.

The skin sensitization potential of 4 -methoxyphenylacetone was estimated by SSS (2018) using OECD QSAR toolbox v3.3 with log kow as the primary descriptor. 4 -methoxyphenylacetone was predicted to be not sensitizing to the skin of female guinea pigs.

Skin sensitization effects were also estimated by four different models i.e, Battery, Leadscope, SciQSAR and CASE Ultra used within Danish QSAR database for 4 -methoxyphenylacetone. Based on estimation, no skin sensitization reactions were observed in guinea pigs and humans. Therefore, 4-methoxyphenylacetone was considered to be not sensitizing.

The experimental and estimated results are in agreement with each other indicating a strong possibility ofp-methoxyphenylacetone being not sensitizing to skin.

These results are supported by the experimental study summarized in Food and Cosmetics Toxicology,12, 929, 1974; for the structurally similar read across substance,4-(p-methoxyphenyl) butan-2-one[CAS: 104-20-1]. Application of 5% in petrolatum was made under occlusion to the skin of 25 human volunteers and observed till 48 hours. 4-(p-methoxyphenyl) butan-2-one produced no skin reactions on 25 human volunteers.

Hence, 4-(p-methoxyphenyl) butan-2 -one is considered to be non-sensitizing to human skin.

The above results are further supported by the experimental study summarized in Toxicology, 9 (1978) 261-271; for the structurally similar read across substance, 4-methoxybenzyl alcohol[CAS: 105-13-5]. The study was performed according to modified Draize sensitization test. The preliminary irritation tests were done in Hartley strain albino guinea pigs to determine concentrations suitable for sensitization testing [injection challenge concentration (ICC) and application challenge concentration(ACC) ] In the induction phase, the total dose was administered on one occasion as 4 intradermal injections, each 2.5 times the ICC (2.5X 0.25). Fourteen days later 10 Inbred Hartley strain albino guinea pigs was challenged intradermally in one flank and topically in the other with 0.1 ml aliquots of test substance at the respective ICC and ACC (0.25 and 10 respectively). Twenty-four hours later the reactions were observed. In the absence of sensitization reactions at first challenge the induction and challenge procedures were repeated, and apparent sensitization reactions confirmed 7 days later by a second challenge with controls included.

Based upon the second challenge, it can be concluded that Anisic alcohol (CAS No: 105-13-5) was considered to be not-sensitizing to the skin of albino Hartley guinea pigs at 0.25% ICC and 10% ACC concentrations.

Based on the available data for the target as well as read across substances and applying the weight of evidence approach,4-methoxyphenylacetone was not sensitizing to skin.Comparing the above annotations with the criteria of CLP regulation, test chemical can be classified under the category “Not Classified”.

Respiratory sensitisation

Endpoint conclusion
Endpoint conclusion:
no study available

Justification for classification or non-classification

Available data for the target chemical, 4-methoxyphenylacetone suggests that it is not likely to cause any dermal sensitization to skin.

4-methoxyphenylacetone can be considered to be not sensitizer to skin and can be classified under the category “Not Classified” as per CLP regulation.