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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from peer reveiwed journals

Data source

Reference
Reference Type:
publication
Title:
Acute Toxicity Studies
Author:
Morton et al
Year:
1975
Bibliographic source:
Toxicology and Applied Pharmacology

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Eye irritation study was conducted to evaluate the irritant nature of Cue-lure to rabbit eyes.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
- Name of test material : 4-(3-oxobutyl)phenyl acetate
-Common Name: Cue-lure
- Molecular formula : C12H14O3
- Molecular weight : 206.2396 g/mol
- Substance type: Organic
- Physical state: Liquid
- Analytical purity: > 98%
- Impurities (identity and concentrations): < 2%
Specific details on test material used for the study:
- Name of test material : 4-(3-oxobutyl)phenyl acetate
-Common Name: Cue-lure
- Molecular formula : C12H14O3
- Molecular weight : 206.2396 g/mol
- Substance type: Organic
- Physical state: Liquid
- Analytical purity: > 98%
- Impurities (identity and concentrations): < 2%

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: The left eye served as control
Amount / concentration applied:
Amount: 0.1mL
Concentration: 100%
Duration of treatment / exposure:
single exposure
Observation period (in vivo):
1, 24, 48, 72 hrs and 7 days
Duration of post- treatment incubation (in vitro):
no data available
Number of animals or in vitro replicates:
6
Details on study design:
TEST SITE
- Area of exposure: conjunctival sac of the right eye
- % coverage: No data available
- Type of wrap if used: No data available

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No data available
- Time after start of exposure: No data available

SCORING SYSTEM: Draize scoring system
The minimum score 0 indicates no irritation observed and maximum score 110 (maximum irritation and damage to the cornea,iris, conjunctivae)

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Score:
0
Max. score:
110
Reversibility:
fully reversible within: 7 days
Remarks on result:
no indication of irritation
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 1 Hour
Score:
12
Max. score:
110
Reversibility:
fully reversible within: 7 days
Remarks on result:
positive indication of irritation
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
24 h
Score:
1.3
Max. score:
110
Reversibility:
fully reversible within: 7 days
Remarks on result:
positive indication of irritation
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
48 h
Score:
0
Max. score:
110
Reversibility:
fully reversible within: 7 days
Remarks on result:
no indication of irritation
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
72 h
Score:
0
Max. score:
110
Reversibility:
fully reversible within: 7 days
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
Some effects were observed in rabbits till 24 hours which got cleared up by 7 days.

Any other information on results incl. tables

Table: Acute Eye Irritation results for Cue-lure

Chemical

Eye irritation score in rabbits*

1 hour

24 hours

48 hours

72 hours

7 days

Cue-lure

12

1.3

0

0

0

*-Draize scoring system; maximum score of 110 is the weighted summation of the grades for the 3 ocular tissues per test, dose – 0.1 ml

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
Some effects were observed in rabbits till 24 hours which got cleared up by 7 days.The mean irritation index for 4-(3-oxobutyl)phenyl acetate[Cue-lure] after 7 days was 0.0.
Hence, 4-(3-oxobutyl)phenyl acetate[Cue-lure] can be considered to be not irritating to rabbit eyes.
Executive summary:

An eye irritation study was conducted to evaluate the irritant nature of 4-(3-oxobutyl)phenyl acetate[Cue-lure] in rabbits.

 

0.1 ml of undiluted sample was instilled into the conjunctival sac of the right eye of each of six albino rabbits. The left eye of each animal served as the control. Grading for eye injury was made at 1, 24, 48 and 72 hr and at 7 days after treatment according to the grading and scoring system of Draize et al. (1944) in which a zero score indicates no irritation and the maximum score at any one scoring period is 110 (maximum irritation and damage to the cornea, iris, and conjunctiva). Some effects were observed in rabbits till 24 hours which got cleared up by 7 days.The mean irritation index for 4-(3-oxobutyl)phenyl acetate[Cue-lure] after 7 days was 0.0.

Hence, 4-(3-oxobutyl)phenyl acetate[Cue-lure]  can be considered to be not irritating to rabbit eyes.