2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

EC Number:

303-085-5

EC Name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Cas Number:

94158-14-2

Molecular formula:

C9H11NO3.ClH

IUPAC Name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Test material form:

solid: crystalline

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

106 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

ln this study AMINOL (A098) was found to be toxic to zebrafish after 96 h at the nominal concentration level of 100 mg/L and higher. The LC50 (96 h) was 106 mg/L (81- 138 mg/L), the NOEC (96 h) was the test item concentration 50 mg/L (all nominal concentrations).

Executive summary:

The acute toxicity of the test item AMINOL (A098) {batch number: R0075037) to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemieals No. 203 (1992) from 2003-08-18 to 2003-08-22 at lhe DR.U.NOACK-LABORATORIUM FüR ANGEWANDTE BIOLOGIE, 31157 Sarstedt, Germany. A static test design was chosen with nominal concentrations of 12.5 - 25 - 50 - 100 - 200 - 400 mg/L. Duration of the test was 96 h. 7 test organisms were exposed to each test concentration and control. Water quality parameters pH-value, temperature and oxygen saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits. The control and test item concentrations of AMINOL (A098) were analytically verified via HPLC-UV at test start and end er whenever 100 % mortality occurred (for results see part 9.2). All effect values are based on the nominal concentrations of the test item. The acute toxicity of the test item AMINOL is summarized in the following table:

Table 1: LC-Values with 95 % Confidence lntervals (based on nominal concentrations)

Test duration [h]	LC50[mg/L]	P=95% [mg/L]
2	>400	Not applicable
24	>400	Not applicable
48	294	89-968
72	106	81-138
96	106	81-138
LC100 Lowest test item concentration with 100% mortality after 96 h	400 mg/L
LC0 Highest test item concentration with 0% mortality after 96 h	25 mg/L
NOEC (0-96 h) No observerd effect concentration *mortality of 1/7 fish is considered not to be relevant	50 mg/L*