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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The biodegradation between both replicates of test item remained in the range of 0-6% until the end of the test. Under the test conditions, the test item is classified as not readily biodegradable in the 10-day-window and within 28 days.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradability of the test item was determined with a non-adapted activated sludge over a test period of 28 days in the modified Sturm test (OECD 301B) and according to GLP. The test item was tested at a concentration of 15 mg/L with 2 replicates, corresponding to a carbon content (TOC) of 10.35 mg C/L in the test vessels. The test vessels were incubated at low light conditions and at a temperature of 21-24°C. The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The percentage CO2 production was calculated in relation to the theoretical CO2 production (ThCO2) of the test item. Functional and toxicity controls were used. All passed the validity criteria. The biodegradation of the test item showed that the mean 10% level (beginning of the biodegradation) was not reached until test end. The biodegradation between both replicates of test item remained in the range of 0-6% until the end of the test. Under the test conditions the test item is classified as not readily biodegradable in the 10-day-window and within 28 days.