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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
ANALOGUE APPROACH JUSTIFICATION
Please refer to the attached read across justification in section 13
Reason / purpose for cross-reference:
read-across source
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Description of key information

The 48 h NOEC of the read-across source substance was determined to be 108 mg/L and the EC50 value was determined to be > 108 mg/L. The EC50 of the target substance is determined to be > 100 mg/L; this value was derived by taking only the active ingredient into account, as the source substance contains approx. 7 % water (reference 6.1.3-1).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
> 100 mg/L

Additional information

No experimental data on short-term toxicity to aquatic invertebrates with the test item was available. Read-across to a structural analogous substance was done instead.


The aquatic toxicity of the source substance was assessed using Daphnia magna in a 48 h Acute Immobilisation Test according to OECD TG 202 and under GLP conditions. For this purpose, young Daphnia were exposed to aqueous test media containing the test item for 48 hours. Based on results obtained in a non-GLP preliminary experiment Daphnia were exposed to a single concentration of 108 mg/L (limit concentration). Potassium dichromate served as the reference control. A static test was performed as the test item was previously shown to be stable in the test medium for 72 h. The purpose of the analytical part of this study was to verify the concentration of the test item in the test medium. The analytically measured test item concentration remained within ± 20 % of the nominal during the test period of 48 h; therefore, biological results are based on the nominal concentration. Healthy, young female daphnids with known origin, history and acclimatization conditions were used. The test item and the control were tested using 20 Daphnia (divided into 4 concurrent subgroups) each. Each test vessel contained approximately 40 mL test medium. Fully characterized (content, physicochemical characteristics) ISO Medium was used as test medium. The immobilisation of the test animals was recorded 24 and 48 h after treatment. Environmental conditions were recorded. All measured values remained within acceptable ranges. All validity criteria were met and therefore the study was considered as valid. The 48 h NOEC of the read-across source substance was determined to be 108 mg/L and the EC50 values were determined to be > 108 mg/L.


Based on the data from the read-across source substance, the target substance's 48 h NOEC and EC50 values were determined to be > 100 mg/L; this value was derived by taking only the active ingredient into account, as the source substance contains approx. 7 % water.