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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
No analytical determination was performed
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
No analytical determination was performed
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
White powder
Specific details on test material used for the study:
Batch n°839411/006Purity : 100,0%Storage : room temperature (20 +/-5 °C), dry

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Variety : StrausStrain : BerlinSex : femaleAge: between 0 and 24 hoursOrigin : Umweltbundesamt BerlinArrival of strain : October 4th 2005

Study design

Test type:
static
Water media type:
freshwater
Remarks:
Deionised water with an enrichment of certain minerals (as demanded in the guideline)
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hours

Test conditions

Test temperature:
23 - 23.2°C
pH:
7.1 - 7.8
Dissolved oxygen:
7.0 mg/L
Nominal and measured concentrations:
Nominal : 1/ 10/ 100 mg/L
Details on test conditions:
Before the study, there is a selection of daphnia. Replciates : Four vessels, each containing 20 mL test solution and five daphnia. Control : Four vessels, each containing 20 mL dilution water and five daphnia. Test vessels glass beakers, nominal volume 50 mL, tall shapeTemperature 20 +/- 2°C
Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
ca. 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 10 - < 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 - < 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L, lyong within the demanded range of 0.6 - 2.1 mg/L.
Reported statistics and error estimates:
All validity critera were met; For the estimation of the EC50 of the reference item, the fit showed sufficient statistical correspondence of the data with the dose-responsa equation

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In accordance the guideline OECD 202, the following results could be determined for the Mephtamin HCL :48h NOEC = 1 mg/L and 48h EC50 = 1 - 10 mg/L
Executive summary:

The experiment was performed using three concentrations ranging from 1 to 100 mg/L. Twenty daphnia were exposed to the test ite for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

In the treatment 100 mg/l, all daphnia were immobilised and 65% were immobilised in the concentration 10 mg/L after 48 hours. None of the animals were immobilised in the control.

The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L, lyong within the demanded range of 0.6 - 2.1 mg/L.

The following results could be determined for the Mephtamin HCL :48h NOEC = 1 mg/L and 48h EC50 = 1 - 10 mg/L