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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the attached read across justification in section 13.
Reason / purpose for cross-reference:
read-across source
Key result
% degradation (O2 consumption)
Sampling time:
28 d

Description of key information

In a study according to OECD TG 301 D under GLP, the source substance was found to be not readily biodegradable. Therefore, the target substance is considered to be not readily biodegradable (reference 5.2.1-1).

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:

Additional information

No experimental data on biodegradation with test item was available. Read-across to a structural analogous substance was done instead.

The read-across source substance was investigated for its ready biodegradability in a Closed Bottle Test according to OECD TG 301 D under GLP conditions over a period of 28 days. The biodegradation was measured by the determination of the oxygen uptake of the microorganisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. Under the test conditions the percentage biodegradation of the test item, based on ThODNH4, reached in the mean -2 % after 28 days of incubation. If the calculation of biodegradation is based on ThODNO3, percentage biodegradation reached in the mean -1 % after 28 days of incubation. The reference item Aniline was sufficiently degraded to 84 % after 14 days, and to 93 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item Aniline, the mean of 36 % biodegradation was noted within 14 days and 46 % biodegradation after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge microorganisms. The degradation rate of the test material did not reach 60 % within the 10day window and after 28 days of incubation. Thus the test item is not readily biodegradable.

Based on the data from the read-across source substance, the target substance is determined to be not readily biodegradable.