Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-775-5 | CAS number: 110-52-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from study report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- other: EWG Directive 84/449/EWG
- Principles of method if other than guideline:
- Determination of primary eye irritation study test chemical in rabbits.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,4-dibromobutane
- EC Number:
- 203-775-5
- EC Name:
- 1,4-dibromobutane
- Cas Number:
- 110-52-1
- Molecular formula:
- C4H8Br2
- IUPAC Name:
- 1,4-dibromobutane
- Test material form:
- liquid
- Details on test material:
- IUPAC name:1,4-dibromobutane
Mol. formula: C4H8Br2
Molecular Weight: 215.915
Smiles: C(CCBr)CBr
InChI: 1S/C4H8Br2/c5-3-1-2-4-6/h1-4H2
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Remarks:
- KFM (SPF quality)
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Kleintierfarm Madoerin AG
CH 4414 Fuellinsdorf/ Switzerland
- Age at study initiation: 14-15 weeks
- Weight at study initiation: 2.3-2.7 kg
- Housing:Individually in stainless steel cage eqiipped with an automatic cleaning and drinking system.
- Diet (e.g. ad libitum): Pelleted stardand Kuba 341, Batch 17/86 rabbit maintenance diet
- Water (e.g. ad libitum):Community tap water from ltingen. ab Libitum.
- Acclimation period:Three days under trest conditions after veterinary examination.
ENVIRONMENTAL CONDITIONS
- Temperature (°C):22±3°C
- Humidity (%):relative humidity 40-70 %
- Air changes (per hr): Air-conditioned with 10-15 air changes per hour.
- Photoperiod (hrs dark / hrs light): 12 hours artificial fluorescent light/12 hours dark, music/light period.
IN-LIFE DATES: From: To: 8 August 1986 to 25 August 1986
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 0.1 ml of test chemical was applied per animal.
- Duration of treatment / exposure:
- No data
- Observation period (in vivo):
- The eyes of each animal were examined 1, 24, 48 and 72 hours, after administration.
- Number of animals or in vitro replicates:
- 3 animals
1 male and 2 females - Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): No data available
- Time after start of exposure: No data available
SCORING SYSTEM: No data available
TOOL USED TO ASSESS SCORE: hand-slit lamp
Results and discussion
In vivo
Results
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.1
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- No staining of the conjuctiva and cornea and no corrosion was observed in the rabbits.
- Other effects:
- - Lesions and clinical observations:
No data
- Ophthalmoscopic findings: No data
- Histopathological findings: No data
- Effects of rinsing or washing: No data
- Other observations:No data
Any other information on results incl. tables
Table 3: Primary irritation score
Selected observations |
Mean scores |
1 hr |
2.7 |
24 hrs |
1.3 |
48 hrs |
0.3 |
72 hrs |
0.0 |
Table 4: Mean scores per animal
Animal & Sex |
Cornea opacity |
Iris |
Conjunctivae |
|
Redness |
Chemosis |
|||
415 M |
0.0 |
0.0 |
0.7 |
0.3 |
146 F |
0.0 |
0.0 |
0.3 |
0.0 |
417 F |
0.0 |
0.0 |
0.0 |
0.0 |
Selected observations for mean: 24 hrs, 48 hrs and 72 hrs
Applicant's summary and conclusion
- Interpretation of results:
- other: Not irritating
- Conclusions:
- The test chemical was found to cause a primary irritation score of 1.1 when applied to the rabbit eye mucosa. No sever effects were observed in 72 hours. Thus, the test chemical was considered to be not Irritating to the eyes.
- Executive summary:
An eye irritation study was performed on 1 male and 2 female New Zealand White KFM (SPF quality) rabbits. The test chemical was applied undiluted at a concentration of 0.1 ml in the conjunctival sac of the left eye of each animal after gently pulling the lower lid away from the eyeball. The lids were then gently held together for about one second to prevent loss of the test article. The right eye remained untreated and served as the reference control. The eyes of each animal were examined 1, 24, 48 and 72 hours, after administration. Body weight was measured at day 1 and at termination of the study. Rabbits were observed for mortality daily. Rabbits were killed by an intraveneous injection of T61 into the ear vein. The irritation was assessed according to the OECD Guidelines for testing of Chemicals, Section 4, number 405 "Acute Eye Irritation/Corrosion". Slight coloration was observed, no corrosive and toxic symptoms were observed in the test animals. Based on obove observations and a primary irritation score of 1.1, the test chemical can be classified as "Not irritant" to the rabbit eye according to the CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
