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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16. Jan 2017 - 14 May 2017
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Details on preparation of test sulution missing
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
A test solution containing 101.8 mg/L in dilution water was prepared
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain/clone: Berlin
- Source: Umweltbundesamt Berlin, Germany
- Age of parental stock (mean and range, SD): <24 h
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
19.7 – 21.1 °C
pH:
7.7
Dissolved oxygen:
8.6 mg/L
Nominal and measured concentrations:
Nominal test substance concentration: 100 mg/L
Calculated test item concentration based on DOC at 0 h: 100.6 mg/L
Calculated test item concentration based on DOC at 48 h: 119.7 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Beakers, glass, nominal volume 50 mL.
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionsed water with an enrichment of certain minerals acc. to guideline


OTHER TEST CONDITIONS
- Photoperiod:16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility of D. magna after 24 and 48 hours

Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none

No immobilised Daphnids observed.
Results with reference substance (positive control):
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (16102802R201). The 24h-EC50i value was determined as 1.8 mg/L, lying within the range of 0.6 – 2.1 mg/L which is mentioned in the OECD guide-line.

Description of key information

EC50 (48 h) > 100 mg/L (nominal)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

The toxicity of the target substance (EC 943-330-9) to aquatic invertebrates was investigated in a study following OECD 202 (GLP). The test organismDaphnia magnawas exposed to a nominal test substance concentration of 100 mg /L in a limit test. The test substance concentration was verified by DOC analysis at test start and test termination after 48 h. The measured concentrations were in a range of 101 % - 120 % of the nominal concentration. Therefore, the effect concentrations were based on the nominal concentrations instead of the measured concentrations. Mortality or behavioural abnormalities were not observed. An EC50 (48 h) > 100 mg/L based on the nominal concentration was reported.