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EC number: 225-803-5 | CAS number: 5089-22-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 13 to November 11, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9408 (Ultimate Aerobic Biodegradability - Method by Determining the Oxygen Demand in a Closed Respirometer)
- Version / remarks:
- 1991
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Amount used, activated sludge: 15 mg/l (dry weight)
- Origin: municipal wastewater treatment plant Frankfurt/M-Sindlingen
- Preliminary treatment: 7 days with stirring.
- Acclimation state: not acclimated in the laboratory to the substance and the reference substance. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 153 mg/L
- Based on:
- test mat.
- Initial conc.:
- 337 mg/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- COD per 1 g of the test material: 2.35 x 10^3 mg/g (O2). The COD per gram of the sample was determined by directly weighing the sample into the COD reaction vessel.
TEST CONDITIONS
- Composition of medium: in accordance with guidelines.
- Test temperature: 22 ± 1 °C
TEST SYSTEM
- Culturing apparatus: BOD bottles were used as the reaction vessel and incubated in the WTW-BSB device.
CONTROL AND BLANK SYSTEM
- Inoculum blank: conducted under the same conditions, witout test material.
- Toxicity control: included. - Parameter:
- % degradation (O2 consumption)
- Value:
- 1 - 5
- Sampling time:
- 28 d
- Details on results:
- The test item was degraded less than 20 %
TOXICITY CONTROL
Control showed no toxicity at the test concentration. - Results with reference substance:
- Oxygen consumption in the reference substance reached more than 60 % of degradation in less than 10 days, after adaptation.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is not readily biodegradable.
- Executive summary:
The assessment of the readily biodegradability potential of the test item was investigated according to the OECD guideline 301F and according to the EU Method C.4-D.
DOC determinations were made in accordance with DIN standard 38 409 part 3 and COD according to the DIN standard 38 409 part 41 and also the requirements of the Directive EEC 84/449. The degradation analysis was carried out by manometric determination of the oxygen consumption.
The reference compound, attained more than 60 % of degradation within 10 days after adaptation, confirming the suitability of the inoculum and culture conditions.
Test item attained negligable degradation within 28 days and therefore cannot be considered as readily biodegradable under the strict terms and conditions of the OECD Guidelines.
Conclusion
The substance is not readily biodegradable.
Reference
Time (d) | Depletion as COD |
7 | 4 |
14 | 5 |
21 | 5 |
28 | 1 |
Description of key information
Not readily biodegradable.
Key value for chemical safety assessment
Additional information
The substance is a bis-benzoxazole derivative; it is not expected to be ready biodegradable because of its chemical structure and the specific function. Commonly, fluorescent brighteners undergo a primary transformation, i.e. discolourization due to the interrupting the conjugation. Nevertheless, the degradation process involves more steps and take more time.
The available experimental results, confirmed that the substance cannot be reapidly biodegraded. The assessment of the readily biodegradability potential of the test item was investigated according to the OECD guideline 301F and according to the EU Method C.4-D. The test item attained negligable degradation (i.e. 1 - 5 %) within 28 days and therefore cannot be considered as readily biodegradable under the strict terms and conditions of the OECD Guidelines.
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