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EC number: 201-803-0 | CAS number: 88-14-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Test chemical concentrations used for the study were 3.125 mg/l, 6.25 mg/l, 12.5 mg/l, 25 mg/l and 50 mg/l,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish
- Source: Anytime Pet
- Length at study initiation (length definition, mean, range and SD): average 1.4 cm
- Weight at study initiation (mean and range, SD): average 0.045 g
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 16 hours light and 8 hours dark
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 155.5 mg of CaCO3
- Test temperature:
- 23.9°C
- pH:
- 7.2
- Dissolved oxygen:
- 7.6 mg/l
- Nominal and measured concentrations:
- Nominal concentrations: 0, 3.125 mg/l, 6.25 mg/l, 12.5 mg/l, 25 mg/l and 50 mg/l, respectively.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Table 1. Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
3.125
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25 4 4 - - - - - - - - - - - - 12.5 4 4 - - - - - - - - - - - - 25 4 4 - - - - - - - - - - - - 50 4 4 - - - - - - - - - - 2 2 Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
3.125
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
50
4 4 NS4 NS4 NS4 NS4 NS4 NS4 NS4 NS4 NS4 NS4 NS2 NS2 Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) was determined to be 50 mg/l.
- Executive summary:
Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.045 g and average length of 1.4 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.6 mg/l, pH 7.5, water temperature 23.9°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test was performed using test chemical concentrations of 0, 3.125 mg/l, 6.25 mg/l, 12.5 mg/l, 25 mg/l and 50 mg/l, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.9°C, pH 7.2, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) was determined to be 50 mg/l. Thus, based on the LC50 value, test chemical was considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be not classified as per the CLP classification criteria.
Reference
Description of key information
Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical (Study report). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.045 g and average length of 1.4 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.6 mg/l, pH 7.5, water temperature 23.9°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test was performed using test chemical concentrations of 0, 3.125 mg/l, 6.25 mg/l, 12.5 mg/l, 25 mg/l and 50 mg/l, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.9°C, pH 7.2, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) was determined to be 50 mg/l. Thus, based on the LC50 value, test chemical was considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 50 mg/L
Additional information
Experimental study of the test chemical and various supporting weight of evidence studies for its read across chemical were reviewed for short term toxicity to fish end point which are summarized as below:
In an experimental study from study report, short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.045 g and average length of 1.4 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.6 mg/l, pH 7.5, water temperature 23.9°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test was performed using test chemical concentrations of 0, 3.125 mg/l, 6.25 mg/l, 12.5 mg/l, 25 mg/l and 50 mg/l, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.9°C, pH 7.2, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) was determined to be 50 mg/l. Thus, based on the LC50 value, test chemical was considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be not classified as per the CLP classification criteria.
In a supporting weight of evidence study, short term toxicity to fish study (from authoritative database, 2018 and secondary source, 1976) was carried out for 96 hrs. Pimephales promelas (Fathead minnow) (Juvenile) of length of 1.1 to 3.1 cm and 4 to 8 weeks old obtained from the Environmental Research Laboratory was used as a test organism. Fish were acclimated in flowing water 11 cm deep in a holding trough (360 cm long by 58 cm wide by 31 cm deep) for at least 48 hr before the tests. Fishes were acclimated in lake superior water. Test organisms were fed Glencoe trout chow no. 3 granules. Three to five widely spaced test concentrations differing by a factor of 10 or more and a control were used. Test solutions were prepared by adding a weighed amount of chemical' to a 9-1. glass carboy containing 4 lit of Lake superior water. Solution components were thoroughly mixed by shaking and were then poured into two glass battery jars for preparation of the toxicant test concentrations. All chemical concentrations are nominal; none were analyzed to determine concentration levels. Test were tested in both lake superior and reconstituted water. The reconstituted water was prepared by adding NaHC03, CaS04. 2H20, MgS04, and KCl salts to distilled water. The static tests were conducted in 3 lit cylindrical glass battery jars containing 2 lit of test water. A glass cover was placed over each jar to reduce evaporation. Test water was not aerated. Test fishes were not fed during the study. Test fishes (20 fish/conc.) were exposed to test chemical for 96 hr at a temperature of 18 to 22°C. Complete immobilization of the fish was considered as the biological endpoint and equated with death. Fish mortality was measured after 1, 24, 48, and 96 hr. Standard graphical procedures were followed for determining concentrations that would result in 50% mortality. On the basis of the effect of test chemical on mortality of the test organism Pimephales promelas (Fathead minnow), the 96 hr LC50 value was determined to be 32 mg/l. Thus, based on the LC50 value, test chemical was considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, chemical was considered as non-toxic to fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria.
For the test chemical from authoritative database (2020), acute toxicity to fish study was carried out. Study was performed following the principles of OECD Guideline 203 (Fish, Acute Toxicity Test). Study was performed under static conditions for a period of 96 hrs. On the basis of the effect of test chemical on mortality of the test organism, the 96 hr LC50 value was determined to be >120 mg/l. Thus, based on the EC50 value, test chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria.
On the basis of the above results, it can be concluded that the test chemical was considered to be toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be not classified as per the CLP classification criteria.
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