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EC number: 943-625-2 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April-May 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)
- Deviations:
- yes
- Remarks:
- no inoculum control used (reference substance e.g. ethylene glycol, aniline)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Physical state: powder
- Analytical purity: >= 95.0% - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: sewage treatment plant Trostberg
- Method of cultivation: aerobic cultivation, dry solids: 5.5g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 680 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 400 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: activated sludge, nutrient medium, deionized water
- Additional substrate: nutrient medium (according to DIN EN 9888)
- Test temperature: 20-25°C
- pH adjusted: yes (to pH 7 +/- 0.5 if required)
TEST SYSTEM
- Culturing apparatus: brown glass vessels (2 L)
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: aeration and agitation
- Measuring equipment: TOC Analyzer Elementar and pH-meter 691, Methrom, Herisau
SAMPLING
- Sampling frequency: at least every 4 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (1 replicate)
- Abiotic sterile control: yes (HgCl2 added, 1 replicate) - Reference substance:
- other: no reference substance used
- Preliminary study:
- Not applicable.
- Test performance:
- No unusual observations during test affecting results.
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 10
- Sampling time:
- 28 d
- Details on results:
- No further details reported.
- Results with reference substance:
- Not applicable, no reference substance tested.
- Validity criteria fulfilled:
- not applicable
- Remarks:
- no test results with reference substance available (e.g. aniline, ethylene glycol)
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- TDI-Urone is not inherently biodegradable under the conditions of the test.
- Executive summary:
TDI-Urone was tested for inherent biodegradability according to the German Guideline DIN EN ISO 9888 which is similar to OECD 302 B (Zahn-Wellens-Test) at an initial concentration of ca. 400 mg DOC/L at temperatures of 20 -25°C for 28 days. Biodegradation was followed by measurements of DOC removal.
Under the conditions of the test, 10 % degradation could be observed which indicates the potential for environmental persistence.
- Endpoint:
- biodegradation in water: ready biodegradability
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
Referenceopen allclose all
Table 1: DOC removal [%]
| DOC removal [%] |
|||
| Date | Day | Test Vessel | HgCl2 Vessel |
| 24.04.2001 | 1 | 9 | 1 |
| 26.04.2001 | 3 | 8 | 5 |
| 30.04.2001 | 7 | 11 | 4 |
| 04.05.2001 | 11 | 11 | 9 |
| 08.05.2001 | 15 | 8 | 3 |
| 11.05.2001 | 18 | 11 | 9 |
| 15.05.2001 | 22 | 16 | 8 |
| 18.05.2001 | 25 | 15 | 10 |
| 21.05.2001 | 28 | 20 | 10 |
Description of key information
Under the conditions of the test guideline IN EN ISO 9888 which is similar to OECD 302 B (Zahn-Wellens-Test), 10 % degradation could be observed. This indicates the potential for environmental persistence.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
TDI-Urone was tested for inherent biodegradability according to the German Guideline DIN EN ISO 9888 which is similar to OECD 302 B (Zahn-Wellens-Test) at an initial concentration of ca. 400 mg DOC/L at temperatures of 20 -25°C for 28 days. Biodegradation was followed by measurements of DOC removal.
Under the conditions of the test, 10 % degradation could be observed which indicates the potential for environmental persistence.
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