Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 943-625-2 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April-May 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)
- Deviations:
- yes
- Remarks:
- no inoculum control used (reference substance e.g. ethylene glycol, aniline)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Physical state: powder
- Analytical purity: >= 95.0% - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: sewage treatment plant Trostberg
- Method of cultivation: aerobic cultivation, dry solids: 5.5g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 680 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 400 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: activated sludge, nutrient medium, deionized water
- Additional substrate: nutrient medium (according to DIN EN 9888)
- Test temperature: 20-25°C
- pH adjusted: yes (to pH 7 +/- 0.5 if required)
TEST SYSTEM
- Culturing apparatus: brown glass vessels (2 L)
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: aeration and agitation
- Measuring equipment: TOC Analyzer Elementar and pH-meter 691, Methrom, Herisau
SAMPLING
- Sampling frequency: at least every 4 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (1 replicate)
- Abiotic sterile control: yes (HgCl2 added, 1 replicate) - Reference substance:
- other: no reference substance used
- Preliminary study:
- Not applicable.
- Test performance:
- No unusual observations during test affecting results.
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 10
- Sampling time:
- 28 d
- Details on results:
- No further details reported.
- Results with reference substance:
- Not applicable, no reference substance tested.
- Validity criteria fulfilled:
- not applicable
- Remarks:
- no test results with reference substance available (e.g. aniline, ethylene glycol)
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- TDI-Urone is not inherently biodegradable under the conditions of the test.
- Executive summary:
TDI-Urone was tested for inherent biodegradability according to the German Guideline DIN EN ISO 9888 which is similar to OECD 302 B (Zahn-Wellens-Test) at an initial concentration of ca. 400 mg DOC/L at temperatures of 20 -25°C for 28 days. Biodegradation was followed by measurements of DOC removal.
Under the conditions of the test, 10 % degradation could be observed which indicates the potential for environmental persistence.
- Endpoint:
- biodegradation in water: ready biodegradability
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
Referenceopen allclose all
Table 1: DOC removal [%]
| DOC removal [%] |
|||
| Date | Day | Test Vessel | HgCl2 Vessel |
| 24.04.2001 | 1 | 9 | 1 |
| 26.04.2001 | 3 | 8 | 5 |
| 30.04.2001 | 7 | 11 | 4 |
| 04.05.2001 | 11 | 11 | 9 |
| 08.05.2001 | 15 | 8 | 3 |
| 11.05.2001 | 18 | 11 | 9 |
| 15.05.2001 | 22 | 16 | 8 |
| 18.05.2001 | 25 | 15 | 10 |
| 21.05.2001 | 28 | 20 | 10 |
Description of key information
Under the conditions of the test guideline IN EN ISO 9888 which is similar to OECD 302 B (Zahn-Wellens-Test), 10 % degradation could be observed. This indicates the potential for environmental persistence.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
TDI-Urone was tested for inherent biodegradability according to the German Guideline DIN EN ISO 9888 which is similar to OECD 302 B (Zahn-Wellens-Test) at an initial concentration of ca. 400 mg DOC/L at temperatures of 20 -25°C for 28 days. Biodegradation was followed by measurements of DOC removal.
Under the conditions of the test, 10 % degradation could be observed which indicates the potential for environmental persistence.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
