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EC number: 231-180-0 | CAS number: 7440-74-6
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxicity studies of heavy metals: lead, bismuth, indium, silver and antimony
- Author:
- Asakura K, Satoh H, Chiba M, Okamoto M, Serizawa K, Nakano M and Omae K.
- Year:
- 2 009
- Bibliographic source:
- J Occup Health, 51(6):498-512
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Remarks:
- GLP compliance not specified in publication
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Zinc chloride
- EC Number:
- 231-592-0
- EC Name:
- Zinc chloride
- Cas Number:
- 7646-85-7
- Molecular formula:
- ZnCl2
- IUPAC Name:
- Zinc dichloride
- Details on test material:
- indium (In, 99%, 45µm, 67246G) kept at room temperature in a dark place until use
Constituent 1
Method
- Target gene:
- not applicable
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 5,000; 1,250; 313; 78.1; 19.5; 4.88; 1.22 and 0.305 µg/plate (dose-finding test)
5,000; 2,500; 1,250; 625; and 313 µg/plate (main test) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- solvent DMSO
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- With S9mix: 2-AA (TA 100; TA 1535, TA 1537, TA98 and WP2µvrA/pKM101. Without S9 mix: AF-2 (TA 100, TA 98 and WP2µvrA/pKM101), NaN3 (TA 1535), 9-AA (TA 1537)
- Positive control substance:
- 9-aminoacridine
- sodium azide
- furylfuramide
- other: 2-AA (2-aminoanthracene)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20min at 37°C
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- The test substance was judged to be mutagnic (or positive) when the mean number of revertant colonies dose-dependently increased two-fold or more than that of the corresponding negative control for at least one tester strain with or without S9mix.
- Statistics:
- the data was not analyzed statistically
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- none
Any other information on results incl. tables
-dose-finding test: the numbers of revertant colonies were less than twice that of the corresponding negative (solvent) control in all tester strains with or without S9 mix.
-main test: the numbers of revertant colonies were less than twice that of the corresponding negative (solvent) control in all tester strains with or without S9 mix.
Applicant's summary and conclusion
- Conclusions:
- The test material was considered to be non-mutagenic for all the used bacterial strains (Salmonella typhimurium as well as Escherichia coli) with as well as without metabolic activation
- Executive summary:
A study was conducted to determine the potential mutagenicity of the test material using bacterial reverse mutation assay (e.g. Ames test).
No significant increases in the frequency of revertant colonies were recorded at any dose level.
The test material was considered to be non-mutagenic under the conditions of this test.
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